目的 观察吡格列酮对缺氧复氧后新生大鼠心肌细胞的保护作用及对蛋白激酶C(PKC)表达的影响.方法 原代培养新生SD大鼠心肌细胞,予吡格列酮、GW9662等预处理24 h后建立缺氧复氧模型.Hoechst33258染色法检测心肌细胞凋亡,免疫印迹方法检测PKC表达.结果 缺氧复氧后心肌细胞早期凋亡率升高(由溶媒对照组的(0.20±0.03)％增加至(12.22±1.45)％,P＜0.05)；吡格列酮减少心肌细胞凋亡(8.32±0.89)％,而吡格列酮抗心肌细胞凋亡的作用可以被GW9662阻断(12.46±1.62)％,P＜0.05；与单纯缺氧复氧组相比,吡格列酮不增加PKC(1.033±0.026,P＞0.05)表达.结论 吡格列酮预处理减轻心肌细胞缺氧复氧后损伤,这种作用部分通过激活PPARγ途径实现；吡格列酮预处理不增加PKC表达.%Objective To investigate the feasibility of vibration test in evaluation of spinal stability after instrument fixation of thoracolumbar burst fracture in pigs. Methods Twenty-one porcine spines (T11 to L5) were impacted longitudinally with different energy levels to simulate thoracolumbar burst fracture. The fractures were fixed by Luque, Harrington, Steffee and Kaneda techniques, and the anterior-posterior, lateral and vertical spinal stability was analyzed using vibration test. Results In the mild injury group, vibration test showed that only the first order frequency showed marked anterior shift in the vertical direction (P<0.05) but the main frequency did not, suggesting loosening between the vertebrae. In moderate- or high-energy impact groups, the main frequency markedly shifted forward in the vertical and anterior-posterior directions (P<0.05 or P<0.01), and obvious lateral shift was observed only in high-energy impact group. Conclusion The dynamical characteristics of the spine indicate that the main frequency markedly shift after instrument fixation (except for Luque) of thoracolumbar burst fracture, suggesting the feasibility of vibration test for evaluating spinal injury severity and instrument fixation.
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