为探讨巴西橡胶树(Hevea brasiliensis)自根幼态无性系与供体间差异产生的原因，应用甲基化敏感扩增多态性扩增技术，对巴西橡胶树体细胞胚发生过程中基因组DNA胞嘧啶甲基化程度和模式进行了分析。结果表明，在巴西橡胶树体细胞胚发生过程中不同阶段的DNA甲基化程度不同，以花药的DNA甲基化程度最高，体细胞胚的DNA甲基化水平最低。在体细胞胚发生过程中出现了I、Ⅱ和Ⅲ3种类型的甲基化多态性带型的改变，包括他们的出现与消失。因此，橡胶树体细胞胚发生过程中可能通过DNA甲基化甲基化和去甲基化来调控基因的表达。%In order to understand the causes of differences between self-rooting juvenile clone and donors of Hevea brasiliensis, the extent and pattern of cytosine methylation in genomic DNA ofH. brasiliensiswere studied by using methylation-sensitive ampliifcation polymorphism (MSAP). The results showed that MSAP proifles of genomic DNA were different during somatic embryogenesis. The genomic DNA methylation of anther was the highest among all tissues, while that of somatic embryo was the lowest. Changes in types of MSAP bands among different tissues were frequently observed, including appearance and disappearance of types I, II, and III MSAP bands. Therefore, the gene expression could be regulated by DNA methylation or demethylation during somatic embryogenesis ofH. brasiliensis.
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