Objective To explore the role of hypoxia-inducible factor 1α(HIF-1α)and carbonic anhydrase IX (CAIX)in oncogenesis and development of gestational trophoblastic disease (GTD).Methods The expressions of HIF-αand CAIX were determined immunohistochemically by specific antibodies on formalin-fixed paraffin sections of normal pregnancy villi (32 cases),hydatidiform moles (33 cases),and invasive hydatidiform moles (26 cases). Results The positive rate of HIF-1α in normal pregnancy villi (NP),hydatidiform moles (HM)and invasive hydatidiform moles (IM) was 28.1% (9/32 ), 45.5% (1 5/33 ) and 73.1% (1 9/26 ), respectively. It was significantly higher in IM than in HM and NP(P <0.05),and there was no statistical difference between the latter two groups (P >0.05).The positive rate of CAIX in NP,HM and IM was 31.2 (10/32),3.0(1/33)and 57.7%(1 5/26),respectively.It was significantly higher in IM than in HM and NP (P < 0.05 ),and CAIX was almost negatively expressed in HM group,which differed significantly from that in NP group (P <0.05 ).There was no correlation between HIF-1αand CAIX expressions in IM (P >0.05).Conclusion The expressions of HIF-1αand CAIX were both higher in gestational trophoblastic neoplasia than in normal pregnancy and hydatidiform moles, which indicates that HIF-1αand CAIX may play a role in oncogenesis and development of malignant transformation of trophoblastic cells.%目的：探索缺氧诱导因子-1α(HIF-1α)和碳酸酐酶 IX(CAIX)在妊娠滋养细胞疾病发生、发展中的作用。方法应用免疫组化 S-P 法检测32例正常早孕绒毛(NP)、33例葡萄胎(HM)和26例侵袭性葡萄胎(IM)组织中HIF-1α和 CAIX 的表达情况。结果HIF-1α在 NP、HM 和 IM 中的阳性表达率分别为28.1%(9/32)、45.5%(15/33)和73.1%(19/26)。在 IM 中的表达水平明显高于 HM 组和 NP 组，差异具有统计学意义(P <0.05)；HM 组与NP 组差异无统计学意义(P >0.05)。CAIX 在 NP、HM 和 IM 中的阳性表达率分别为31.2%(10/32)、3.0%(1/33)、57.7%(15/26)。在 IM 中的表达水平明显高于 HM 组和 NP 组，差异具有统计学意义(P <0.05)；在 HM 组织中几乎呈阴性表达，与 NP 组 差 异 具 有 统计学意义 (P <0.05)。 HIF-1α和 CAIX 在 IM 中的表达无显著性关联(P >0.05)。结论HIF-1α和 CAIX 的表达水平随着滋养细胞恶性程度的增加而明显升高，提示两者可能与妊娠滋养细胞疾病的发生发展及恶性转化有关。
展开▼
