目的 研究甲基苯丙胺(methamphetamine,MA)急性处理对LPS诱导的小鼠脾脏IL-6、IL-10和TNF-α表达的影响.方法 选用C57BL/6J小鼠,分别腹腔注射(intraperitoneally,i.p.)生理盐水(NS)或MA(5 mg/kg),10 min后,再分别i.p.NS和LPS(150 μg/kg),予LPS后30 min和60 min,取脾脏,检测这两个时间点小鼠脾脏IL-6、IL 10和TNF-α的水平.结果 MA可显著提高LPS诱导的脾脏IL-6和IL-10的表达(P＜0.01).对于脾脏IL-6的水平存在MA+ LPS的交互效应(P＜0.01),在i.p.LPS60min后MA+ LPS组脾脏IL-6水平较NS+ LPS组明显升高(P＜0.01).对于脾脏IL -10的水平也存在MA+ LPS的交互效应(P＜0.01),在i.p.LPS30min后MA+ LPS组脾脏IL-10水平较NS+ LPS组明显升高(P＜0.01).对于TNF-α,在这两个时间点既无显著的MA的主效应,也无MA+ LPS的交互效应,MA组较NS组几乎没有差异(P=1.00).同一组别两个时间点的比较发现,无LPS刺激,小鼠脾脏IL-6、IL-10和TNF-α的水平在两个时间点无差异；给予LPS刺激,i.p.LPS后60 min小鼠脾脏IL-6、IL-10和TNF-α的水平较30 min显著增高(P＜0.01).结论 MA处理小鼠后短时间内可显著增加LPS诱导的脾脏IL-6和IL-10的表达,但对TNF-α表达无影响.%Objective To study the effects of acute methamphetamine (MA) exposure on lipopolysaccharide (LPS)-induced spleen cytokines IL-6, IL-10 and TNF-a in mice. Methods C57BL/6J mice were injected intraperitoneally with either MA (5 mg/kg) or normal saline (NS) (0. 1 mL/kg); 10 min following MA administration, the mice were given an immune stimulus of LPS (150 f*g/kg) and their spleens were collected 30 min and 60 min after LPS treatment. Spleen samples were assessed for IL-6, IL-10 and TNF-a levels using ELISA kits. Results MA alone could increase the expressions of IL-6 and IL-10 (P<0.01). For IL-6 level, there was an MA + LPS interaction (P<0.01) whereby MA increased the spleen level of IL-6 compared with LPS alone at 60 min post-LPS (P<0.01). There was also an MA+LPS interaction for IL-10 (P<0.01) where MA increased the spleen level of IL-10 compared with LPS alone at 30 min post-LPS (P<0. 01). For TNF-a, there was no significant difference between MA treatment group and control group at 30 min and 60 min post-LPS (P = 1.00). Comparison between the two different time points showed that the expressions of IL-6, IL-10 and TNF-a in the same group with LPS stimulation increased significantly at 60 min post-LPS (P<0.01), whereas the levels of these cytokines without LPS stimulation did not differ significantly. Conclusion Acute MA exposure can significantly increase the expressions of LPS-induced spleenocytokines IL-6 and IL-10; however, it does not affect TNF-a expression.
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