Objective To study the effects of miRNA-449a/b on apoptosis of gastric cancer cells and expression of its target CCNE2. Methods The expression of miRNA-449a/b in gastric cancer cell line BGC823 after transient transfection was detected by Real-time PCR. Flow cytometry was used to detect cell apoptosis rate. The target gene of miR-449a/b was detected by Western blot. Results At 48 and 72 h, miRNA-449a/b was overexpressed in transient transfection cells and the upregulated miRNA-449a/b could inhibit proliferation and promote apoptosis (P<0.05). The expression of CCNE2 was also inhibited significantly (P<0.05). Conclusion miRNA-449a/b inhibits gastric cancer cell probably by targeting CCNE2.%目的 研究miRNA-449在胃癌细胞中对细胞增殖、凋亡的影响,以及对靶基因CCNE2( cyclin E2)表达的影响.方法 荧光定量PCR检测人低分化胃癌细胞株BGC823瞬时转染miRNA-449a/b及抑制剂后的表达;流式细胞术分析转染后细胞凋亡变化.Western blot检测miRNA-449a/b对靶基因表达的影响.结果 在转染后,miRNA-449a/b组在48、72h对肿瘤细胞的增殖有明显的抑制作用(P＜0.05),miRNA-449a/b组对肿瘤细胞凋亡有明显的促进作用(P＜0.05),对CCNE2蛋白表达有显著抑制作用(P＜0.05).结论 miRNA-449a/b可能通过CCNE2来发挥其抑制胃癌细胞生长的作用.
展开▼
