Objective To investigate the clinic distribution and drug resistance of Pseudomonas aeruginosa and to offer reliable basis in using reasonable antibiotic. Methods All the Pseudomonas aeruginosa strains were identified by the automated analyzer VITEK-32 system, and its antibiotic resistance was determined with K-B method recommended by Clinical and Laboratory Standards Institute and analyzed by WHONET 5. 4 software. Results Three hundred and eighty-two strains of Pseudomonas aeruginosa were mainly distributed in intensive care unit(36. 9% ) and isolated from sputum(69. 9% ). The resistance rate of Pseudomonas aeruginosa to 18 antibiotics shown an increasing trend; the antibiotics with below 30. 0% drug-resistance rate include:polymyxin E, ciprofloxacin,levofloxacin, imipenem, meropemem,ceftriaxone and ceftazidime;and that over 50.0% were as followed; gentamycin, ticarcillin, ampicillin, and compound sulfamethoxazole. Conclusion Drug-resistance status of Pseudomonas aeruginosa are very serious in local hospital. We should strengthen the drug resistance survillence and choose antimicrobial agents reasonably according to the antimicrobial susceptibility test.%目的 调查铜绿假单胞菌的临床分布及耐药性变迁,为临床合理选用抗菌药物提供依据.方法 采用VITEK-32全自动微生物鉴定系统鉴定细菌,参照美国临床和实验室标准化协会推荐的方法,应用K-B法进行药物敏感(药敏)试验,用WHONET 5.4软件分析铜绿假单胞菌的耐药性.结果 382株铜绿假单胞菌主要分布在重症监护病房(36.9％),标本主要来自痰液标本(69.9％)；药敏试验结果显示:铜绿假单胞菌对18种临床常用抗菌药物的耐药率呈逐年上升的趋势；耐药率＜30.0％的抗菌药物有多黏菌素E、环丙沙星、左氧氟沙星、亚胺培南、美罗培南、头孢曲松和头孢他啶；耐药率＞50.0％的有庆大霉素、替卡西林、氨苄西林和复方新诺明.结论 铜绿假单胞菌耐药性比较严重,应对其加强耐药性监测,根据药敏试验结果合理谨慎地使用抗菌药物.
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