[目的]研究穿山龙总皂苷对哮喘小鼠气道重塑和平滑肌肌动蛋白（α-SMA）的影响。[方法]SPF级BALB/c小鼠50只随机分为空白组、模型组、穿山龙总皂苷高剂量组、中剂量组、低剂量组。卵蛋白（OVA）致敏激发小鼠制作哮喘气道重塑模型，在实验第18-55d，空白组、模型组以生理盐水灌胃，药物组分别给予穿山龙总皂苷80mg·kg-1、40 mg·kg-1、20 mg·kg-1治疗。实验结束后HE染色观察哮喘小鼠肺组织病理形态变化，采用免疫组织化学法检测α-SMA水平。[结果]穿山龙总皂苷能改善哮喘小鼠气道壁及支气管平滑肌的增厚；与空白组比较，模型组小鼠α-SMA明显升高（P<0.05）；与模型组比较，高、中剂量组α-SMA水平明显降低（P<0.05）。[结论]穿山龙总皂苷能够抑制哮喘小鼠肺组织表达α-SMA，影响平滑肌增厚，改善哮喘小鼠气道重塑状态。% [Objective]To Study the effect of Con-saponin of Dioscoreae Nipponicae(CDN) on expressions of airway remodeling and smooth muscle actin-α(α-SMA)in asthmatic mice. [Method] Fifty SPF BALB/c mice were randomly divided into normal group, model group, high-dose CDN group, medium-dose CDN group, low-dose CDN group. The five groups except normal group were induced as asthmatic modeling al ergies mice with ovalbumin(OVA).During the 18~55 days in the experiment, the normal and model groups were treated with normal saline. Meanwhile, CDN groups were respectively treated with 80 mg·kg-1, 40 mg·kg-1, 20 mg·kg-1 of CDN. At the end of experiment, we observed the path-morphological changes in lung issues and took the sample blood to detect α-SMA using IC. [Result]The pathological changes in lung tissue showed that CDN could significantly inhibit the thickening of airway wel and bronchial smooth muscle. The level of α-SMA was significantly increased except the normal group(P<0.05).In high-dose and medium-dose CDN groups, the level of α-SMA was significantly decreased of α-SMA which reduced bronchial smooth muscle thickening and suppressed airway remodeling in asthmatic mice.
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