[目的]通过流式细胞术检测人类白细胞抗原B27 (HLA-B27)在各类疾病患者中的表达,评价HLA-B27的临床应用价值.[方法]用流式细胞仪分析2008年1月1日至2011年6月27日杭州各大医院4272例入院就诊患者外周血T淋巴细胞HLA-B27抗原表达.[结果]HLA-B27表达阳性有1209例,另有5例阳性结果需进一步确认,阳性率为28.30％,其中男性阳性率为34.58％,,女性为20.36％;阳性患者中主要以21岁至40岁为主,按年龄组分成≤20、21～30、31～40、41～50、≥50岁,男性阳性率分别为11.76％、36.12％、26.42％、13.94％和11.76％,女性分别为7.03％、25.78％、32.03％、15.36％、20.31％;强直性脊柱炎(AS)、类风湿性关节炎(RA)、风湿病、腰椎间盘突出症等疾病HLA-B27表达阳性率分别为89.25％、54.55％、47.82％、20.75％.[结论]HLA-B27阳性表达率男性高于女性,主要集中于21～40岁,且与AS具有高度相关性.%[Objective] The expression of human leukocyte antigen B27 (HLA-B27) in patients with various diseases was detected by Flow Cytometry to evaluate the clinical value of HLA-B27. [Methods] The expression of HLA -B27 antigen of peripheral blood T lymphocytes in 4272 hospital admission patients was analyzed by Flow Cytometry. [Results] 1209 patients were positive in the 4272 hospital admission patients, and another five patients with positive results need further confirmation. The HLA-B27 positive rate was 28.30%(1209/4272), of which men was 34.58%(825/2386) and women was 20.36%(384/1886). The age of the HLA -B27 positive patients was mainly between 21 and 40 years old. Composed by age ≤ 20,21~ 30,31 ~40,41~50,≥50 years old, the positive rates of male and female were 11.76%, 36.12%, 26.42%, 13.94%, 11.76%(97,298, 218,115,97/825), [7.03%, 25.78%, 32.03%, 15.36%, 20.31% (27,99,123,59,78 / 384)]. The positive rates of HLA-B27 expressing in ankylosing spondylitis (AS), rheumatoid arthritis (RA), rheumatoid arthritis and lumbar disc hemiation patients were 89.25% (83/93), 54.55% (36/66), 47.82% (11/23) and 20.75% (22/106), respectively. [Conclusion] The positive patients of HLA-B27 were higher in men than women, and mainly focused on 21 to 40 years old, and the HLA-B27 positive rate was highly correlated with AS.
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