目的 了解SLCO1B1和载脂蛋白E(ApoE)基因单核苷酸多态性在华南地区汉族人群的分布情况.方法 采用聚合酶链反应(PCR)-荧光探针技术对142例心血管疾病患者和42例健康对照者的SLCO1B1和ApoE基因多态性进行检测,并统计男女间及患者组与健康对照组间的基因型分布差异.结果 SLCO1B1的*1a/*1a+*1a/*1b+*1b/*1b组合最多见,占73.2%(104/142),各基因型组合在男女人群间的分布差异无统计学意义(P>0.05),患者组与健康对照组间的分布差异也无统计学意义(P>0.05);A po E以E2/E4+E3/E3组合最为多见,占78.9%(112/142),各基因型组合在男女人群间的分布差异无统计学意义(P>0.05),但患者组与健康对照组间的分布差异有统计学意义(P<0.05).结论 SLCO1B1和ApoE的基因多态性分布与性别无关,但A po E的多态性在心血管疾病患者和健康人群中分布有差异.%Objective To understand the distribution situation of single nucleotide polymorphism of SLCO 1B1 and ApoE genes among Han population in south China area .Methods The PCR fluorescence probe technique was adopted to detect SLCO 1B1 and ApoE genetic polymorphisms in 142 patients with cardiovascular disease and 42 healthy controls .The differences of genotypes dis-tribution between males and females and between the patients and healthy controls were statistically analyzed .Results The combi-nations of SLCO1B1*1a/*1a+ *1a/*1b+ *1b/*1b were most common ,accounting for 73 .2% (104/142) ,the distribution of each genotype combination had no statistically significant difference between male and female populations (P>0 .05) ,the distribu-tion also had no statistically significant difference between the patients group and healthy control group (P>0 .05);the combination of ApoE E2/E4+E3/E3 was most common ,accounting for 78 .9% (112/142) .The distribution of each genotype combination had no statistically significant difference between male and female populations (P>0 .05) ,but the distribution had statistically signifi-cant difference between the patients group and healthy control group (P<0 .05) .Conclusion SLCO1B1 and ApoE polymorphism distribution has no relation with gender ,but ApoE polymorphism distribution has difference between the cardiovascular patients and healthy people .
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