Objective To construct a highly specific and highly sensitive detection techique for the real‐time online detection of platelet‐derived growth factor (PDGF) based on nucleic acid aptamer and nanogold (AuNPs) sig‐nal amplification technology by using surface plasmon resonance (SPR) microarray sensor .Methods 1 ,4‐benzene 2 mercaptan was coupled on the biochip surface by self‐assembly method .Then the modified biochip with gold nanop‐articles ,and immobilized PDGF aptamer screened by SELEX technology on the SPR biosensor micro array as a de‐tecting probe was put in the real‐time online analysis system for detecting the PDGF concentration in the solution . The effect of relevant experimental conditions ,including the concentration of aptamer and immobilization conditions were investigated and optimized ,and the stability of this detection method and the linear range were further studied . Results This novel rapid PDGF detection method realized the real time detection of PDGF with a good stability of the detection platform and a good linearity in the PDGF concentration range of 0 .1-50 μmol/L ,its lowest detection limit was 0 .02μmol/L .At the same time the detection system had the multichannel parallel detection ability without mutual interference .Conclusion This rapid aptamer PDGF detection technique based SPR biosensor possesses the advantages of high sensitivity and good stability ,which has broad development prospect in the clinical diagnostic work .%目的：构建一种基于核酸适配子型表面等离子体共振（SPR）传感器微阵列与纳米金（AuNPs）信号放大技术的高特异性、高灵敏度的可实时在线检测血小板源性生长因子（PDGF）的检测技术。方法利用1，4‐苯二硫醇通过自组装技术耦联于生物芯片表面，然后在生物芯片表面修饰金纳米颗粒，将采用系统配体进化技术（S E L‐EX）筛选得到的PDGF适配子处理后固定于SPR生物传感器微阵列上成为检测探针分子，然后将微阵列置于实时在线分析系统上对溶液中的PDGF浓度进行实时在线检测；并优化固定的核酸适配体浓度和固定条件，进一步研究了该检测方法的稳定性与线性检范围。结果该新型快速检测方法能够实现对PDGF的快速实时检测，稳定性好；在0．1～50μmol／L PDGF的范围内具有较好的线性，且最低检测下限可达0．02μmol／L。结论该基于适配体的SPR传感器技术具有灵敏度高、稳定性好等优点，在临床诊断工作中有着广阔的发展前景。
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