Objective To investigate the change of peripheral blood CD4+ and CD8+ T lymphocytes and its clinical significance in the patients with multiple inflammatory diseases such as malignant tumors ,tuberculosis ,Si-ogren syndrome ,uveitis and systemic inflammatory response syndrome (SIRS ) .Methods The peripheral blood CD4+ ,CD8+ T lymphocytes in the patients with above diseases were detected by the BD FACSCanto II flow cyctome-try and the ratio of CD4+ /CD8+ T cells was calculated .At the same time 20 individuals with healthy physical exami-nation as the healthy control group were measured .The detection results were statistically analyzed .Results Com-pared with the healthy control group ,peripheral blood CD4+ T lymphocytes and the ratio of CD4+ /CD8+ in the ma-lignant tumors group were significantly decreased with statistical difference between the two groups (P<0 .05);pe-ripheral blood CD4+ T lymphocytes and the ratio of CD4+ /CD8+ in the SIRS group were significantly decreased and CD8+ T lymphocytes were increased compared with the healthy control group ,the difference had statistical signifi-cance between the two groups(P<0 .05);CD8+ T lymphocytes in the uveitis group were increased compared with the healthy normal controls ,the difference had statistical significance(P<0 .05);CD4+ T lymphocytes in the pulmonary tuberculosis group were decreased ,the difference had statistical significance(P<0 .05) .Conclusion The abnormality of peripheral blood CD4+ ,CD8+ T lymphocytes in the patients means the damage of cellular immune function .The CD4+ and CD8+ T lymphocytes detected by flow cytometry can be considered as the preliminary diagnostic indicators of the immune function ,which can assist the disease treatment and condition observation .%目的：探讨恶性肿瘤、肺结核、干燥综合征、葡萄膜炎等多种炎性疾病患者外周血CD4＋和CD8＋淋巴细胞的变化及其临床意义。方法应用BD FACSCanto II流式细胞仪检测上述疾病患者外周血CD4＋、CD8＋ T细胞并计算两者比值，同时检测健康者标本20例作为健康对照组，进行统计分析。结果与健康对照组比，恶性肿瘤组CD4＋ T淋巴细胞、CD4＋/CD8＋均明显降低，CD8＋ T淋巴细胞增高，差异有统计学意义（ P＜0．05）；全身炎性反应综合征组CD4＋ T淋巴细胞、CD4＋/CD8＋均明显降低，CD8＋ T 淋巴细胞增高，差异有统计学意义（ P＜0．05）；葡萄膜炎组CD8＋ T淋巴细胞增高，差异有统计学意义（P＜0．05）；肺结核组CD4＋ T淋巴细胞降低，差异有统计学意义（ P＜0．05）。结论患者的 CD4＋、CD8＋ T 淋巴细胞异常，表明机体细胞免疫功能异常。流式细胞仪检测CD4＋、CD8＋ T淋巴细胞可以作为患者临床免疫功能的初步诊断指标，辅助疾病治疗和病情观察。
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