为了研究鼠疫耶尔森氏菌(Y. pestis) 保护性抗原V蛋白，从基因库中查得Y. pestis LcrV基因DNA序列，针对序 列设计合成了一对PCR扩增引物，以本所保存的Y. pestis菌种为模板进行 基因扩增，结果获得长约980 bp的DNA片段。将扩增产物回收纯化，克隆至pGEMT载体，构 建重组载体pGEMT/ypV, 经过PCR，酶切鉴定，并对pGEMT/ypV中的V基因片段进行测序， 分析测序结果与已知序列相同，表明获得了LcrV基因。%To study the protective antigen V protein of Yersinia pestis, a pair of primers for PCR were designed according to published DNA sequence of Y. pestis LcrV gene and synthesized. A DNA fragment about 980 bp length was gotten through PCR amplification. After re cycled and purified, the DNA fragment was ligated with pGEMT Vector and recomb ined vector pGEMT/ypV was constructed. The pGEMT/ypV plasmid was then identi fied by PCR, enzyme digestion and sequencing, and the sequence was the same as t he published sequence.
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