目的：对公共场所环境样本（水、土壤、气溶胶）进行嗜肺军团菌监测，掌握南京市公共场所中军团菌的污染情况和菌型分布。方法：对12家商场、医院中央空调系统采集水样（冷却水、景观水、淋浴水、自来水），土壤类样品（背景土、花卉土、风管积尘、景观土），采用传统细菌培养法及荧光PCR法进行嗜肺军团菌的分离鉴定。结果：12家单位共采样234份（水样88份、土壤146份），传统细菌培养法检出嗜肺军团菌9份（主要类型为Lp1型），检出率为3.85%；而实时荧光PCR法检出47份阳性（主要类型为Lp1型），检出率为20.08%。两者检出阳性率比较差异有统计学意义（P<0.05）。结论：南京市公共场所存在嗜肺军团菌污染，荧光PCR法总检出率明显高于传统细菌培养法，荧光PCR方法较培养法灵敏性较好。%Objective:To monitor the Legionella pneumophila of public environmental samples(water,soil, aerosol),and grasp the pollution situation and strains of Legionella pneumophila in public places in Nanjing city distribution.Method:Samples in water and soils of twelve stores and hospitals were collected,Legionella pneumophila were detected and identified by tradtional culture and real-time PCR. Result:234 samples were collected(water 88 samples,soil 146 samples)and 9 Legionella pneumophila strains were isolated used culture methods(the main type of Lp1 type),the detection rates of pneumophila was 3.85%. while 47 samples were tested positive using Real-time PCR (the main type of Lp1 type). The detection rates of pneumophila were 20.08%. The detection rate of positive comparative difference was statistically significant(P<0.05). Conclusion:Legionella pneumophila are existed in public places of Nanjing. Legionella pneumophila detection rate is higher by Real-time PCR than traditional culture,and it is more sensitive than culture methods.
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