本文旨在研究有氧运动加螺旋藻补充对2型糖尿病大鼠肾脏TLR4/NF-κB-p65的表达及炎性因子的影响,以TLR4/NF-κB-p65炎症信号通路为靶点探讨运动加螺旋藻补充改善2型糖尿病大鼠肾脏损伤的可能机制.采用4周高脂饲料喂养和低剂量STZ腹腔注射的方法建立2型糖尿病大鼠实验模型,成模后将大鼠随机分为糖尿病安静对照组(DM)、糖尿病运动组(DE)、糖尿病+螺旋藻组(DS)、糖尿病运动+螺旋藻组(DES),另设正常安静对照组(NC).运动方式采用8周的无负重游泳训练.实验末,测随机血糖、微量白蛋白、肾脏测TNF-α、TLR4和NF-κB-p65蛋白表达,并采用光镜观察肾脏微细结构的改变.结果表明:(1)光镜下可见DE组、DS组和DES组大鼠肾小球毛细血管袢面积增加;肾小管扩张、上皮细胞空洞变性、细胞聚集增加和炎症细胞稍浸润等病理变化均较DM组有所改善.(2)DE组、DS组和DES组大鼠的血糖浓度、尿微量白蛋白含量均较DM组降低(P＜0.05或P＜0.01);与DES组比较,DE组和DS组的血糖浓度、尿微量白蛋白含量均升高明显(P＜0.05或P＜0.01).(3)DE组、DS组和DES组大鼠肾脏TNF-α、TLR4和NF-κB-p65蛋白表达均明显降低,与DM组比较,差异均呈显著性(P＜0.01);与DES组比较,DE组和DS组的肾脏TNF-α、TLR4和NF-κB-p65蛋白表达明显增加(P ＜0.05或P＜0.01).因此,有氧运动、螺旋藻及两者联合使用有降低2型糖尿病大鼠血糖,改善肾功能,减轻肾脏损害的功效,但两者联合使用效果要好于单独使用,其机制可能与有氧运动联合螺旋藻降低TLR4/NF-κB-p65信号蛋白表达发挥抗炎作用的效果要好于单纯的有氧运动或螺旋藻使用.%To investigate the effects and possible mechanism of aerobic exercise and spirulina supplement on the protein expression of TLR4/NF-κB-p65 as a target of improving renal injury and inflammatory factor in kidney tissue of type 2 diabetic rats.The model of type 2 diabetic rats was established through SD rats fed high-fat diet for four weeks together with intraperitoneal infecting a low dose of STZ.The model rats were randomly divided into diabetic control group(DM) and diabetic exercise group (DE) with intervention of swimming training for eight weeks,and diabetic plus spirulina group(DS) and diabetic exercise plus spirulina group(DES),additionally provided normal control group (NC) without any intervention.The blood glucose concentrations and the excretion of 24 h microalbuminuria(UAE) were examined,and the expressions of TNF-α,TLR4 and NF-κB-p65 in the renal cortex and glomerular microstructure changes were observed by light microscope after the experiment end.The results indicated that group DM had pathological changes of glomerular capillary loop increased and dilation of renal tubule and its epithelial cell epithelial and degeneration.In addition,cell aggregation increased and infiltration of inflammatory cells,while the above glomerular injury improved significantly in groups of DE,DS and DES with exercise and spirulina supplement through light microscope.Compared with group DM,the concentrations of blood glucose and 24h UAE in the intervention groups decreased significantly(P ＜ 0.05 or P ＜ 0.01),and that improved significantly after two interventions than the single intervention.Compared with group DM,the expressions of NF-κB-p65 and TNF-α,TLR4 significantly decreased in group DE of the renal cortex(P ＜0.05 or P ＜ 0.01),and that improved significantly after two interventions than the single interventions.Therefore,exercise and/or spirulina supplement can improve blood glucose and renal function,and decrease renal injure for type 2 diabetes.Combination of swimming and spirulina supplement showed stronger effects on protein expression of TLR4/NF-κB-p65 signal which had anti-inflammatory effect than either sole swimming or sole spirulina supplement.
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