研究槐角的化学成分及其抗骨质疏松作用.利用硅胶柱、ODS反相硅胶柱、Sephadex LH-20凝胶柱和半制备型高效液相等色谱技术对槐角进行分离纯化,并结合化合物的理化性质和波谱数据确定化合物的结构.从槐角中分离得到15个化合物,分别为染料木素(1)、山萘酚(2)、鸢尾苷(3)、芒柄花苷(4)、α-鼠李异洋槐素(5)、鹰嘴豆芽素A-7-O-β-D-葡萄糖苷(6)、南酸枣苷(7)、thevetiaflavon(8)、香豌豆酚-7-O-β-D-葡萄糖苷(9)、山萘酚-7-O-β-D-葡萄糖苷(10)、槲皮苷(11)、山萘酚-3-O-β-D-槐糖苷(12)、染料木素-7,4'-双葡萄糖苷(13)、槲皮素-3-O-β-D-槐糖苷(14)、山萘酚-3-O-β-D-槐糖-7-O-α-L-鼠李糖苷(15).其中化合物6～12、14为首次从该种植物中分离得到,化合物7、8、10、14为首次从该属植物中分离得到.对所分离的化合物进行了抗骨质疏松体外药理活性筛选,结果表明除化合物7和15以外,其他化合物均具有促进成骨细胞增殖作用.%To study the chemical constituents from Fructus Sophorae,the dry mature fruits of Sophora japonica L..The constituents were separated and purified by silica gel column chromatography,ODS revemed phase column chromatography,Sephadex LH-20 column chromatography and semi-preparative HPLC.Their structures were identified by physical and chemical properties and spectral data.Fifteen compounds were isolated from Fructus Sophorae,and identified as genistein (1),kaempferol (2),tectoridin (3),ononin (4),α-rhamnoisorobin (5),sissotrin (6),choerospondin (7),thevetiaflavon (8),orobol-7-O-β-D-glucoside (9),kaempferol-7-O-β-D-glucoside (10),quercitrin (11),kaempferol-3-O-β-D-sophoroside (12),genistein-7,4'-O-β-D-diglucoside (13),quercetin-3-O-β-D-sophoroside (14),kaempferol-3-O-β-D-sophoroside-7-O-α-L-rhamnoside (15).Compounds 6-12 and 14 were obtained from this plant for the first time.Compounds 7,8,10 and 14 were obtained from genus Sophora Linn.for the first time.The isolated compounds were screened for anti-osteoporosis activities,and the results suggested that Fructus Sophorae can be an excellent source of natural antiosteoporosis agents.The results showed that all compounds can promote osteoblast proliferation except compounds 7 and 15.
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