在筛选微生物来源代谢产物的过程中,分离得到一株正青霉Eupenicillium sp.E-UN41,发酵液经离子交换树脂和葡聚糖凝胶等方法分离得到化合物UN41A.通过核磁共振波谱和X-单晶衍射进行该化合物的分子构型和晶体结构分析,确定其为4-氨甲酰基-1-β-D-呋喃核糖-5-羟基咪唑,与咪唑立宾同质.晶体属斜方晶系,空间群P212121,晶胞参数a=7.4897(7),b=11.3801 (15),c=14.0090(13)(A),V=1194.0(2) (A)3,Z=4,Dc=1.553g/m3,F(000) =592.晶体结构分析表明,分子中存在咪唑环共平面.%Eupenicillium sp.E-UN41 was obtained in the course of screening for bioactive microbial products,the compound UN41A was isolated by column chromatography of ion exchange resin and Sephadex G10,and purified by crystallization.The configuration and stereochemistry structure of UN41A were determined by NMR spectra and single crystal X-ray diffraction.UN41A was proved to be 4-carbamoyl-1-B-D-ribofuranosyl imidazolium-5-olate,the same structure as the immunosuppressant,mizoribine.The crystal of UN41A bebnged to the orthorhombic P212121 space group with a =7.4897(7),b =11.3801 (15),c =14.0090 (13) (A),V =1194.0(2) (A)3,Z =4,Dc=1.553g/m3,F (000) =592.The crystal of UN41A revealed that there was a imidazole ring plane.
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