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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Identification, molecular characterization, and structural analysis of the bla( NDM-1 )gene/enzyme from NDM-1-producing Klebsiella pneumoniae isolates
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Identification, molecular characterization, and structural analysis of the bla( NDM-1 )gene/enzyme from NDM-1-producing Klebsiella pneumoniae isolates

机译：来自NDM-1生产Klebsiella肺炎肠分离物的BLA（NDM-1）基因/酶的鉴定，分子表征和结构分析

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摘要

NDM-1 comprises a carbapenemase that was first detected in 2008 in New Delhi, India. Since then, NDM-1-producing Klebsiella pneumoniae strains have been reported in many countries and usually associated with intra and inter-hospital dissemination, along with travel-related epidemiological links In South America, Brazil represents the largest reservoir of NMD-1-producing K. pneumoniae. Here, we focused on the detection and molecular/structural characterization of the bla (NDM-1) resistance gene/enzyme from 24 K. pneumoniae clinical isolates in the Midwest region of Brazil. Antimicrobial susceptibility assays showed that all isolates are resistant to carbapenems. Molecular typing of the isolates revealed seven clonal groups among the K. pneumoniae isolates, which may indicate intra or inter-hospital dissemination. Moreover, the bla( NDM-1) gene was detected in all 24 K. pneumoniae isolates and the full bla (NDM-1) gene was cloned. Bioinformatics analysis showed that the NDM-1 enzyme sequence found in our isolates is highly conserved when compared to other NDM-1 enzymes. In addition, molecular docking studies indicate that the NDM-1 identified binds to different carbapenems through hydrogen and zinc coordination bonds. In summary, we present the molecular characterization of NDM-1-producing K. pneumoniae strains isolated from different hospitals, also providing atomic level insights into molecular complexes NDM-1/carbapenem antibiotics.
机译：NDM-1包含在印度新德里的2008年首次检测到的碳结构酶。从那时起，在许多国家据报道，产生的NDM-1产生的Klebsiella肺炎菌株，通常与医院内部传播相关，以及南美洲的旅行相关的流行病学联系，巴西代表了最大的NMD-1制作水库K.肺炎。这里，我们专注于Bla（Ndm-1）抗性基因/酶的检测和分子/结构表征在巴西中西部地区的24k.pneumoniae临床分离株。抗微生物敏感性测定显示所有分离株均对肉豆蔻蛋白耐药。分子的分子键入分离物揭示了K.肺炎群分离株中的七个克隆基团，其可能表明宫内或医院间传播。此外，在所有24K中检测到BLA（NDM-1）基因。肺炎群分离物和克隆全BLA（NDM-1）基因。生物信息学分析表明，与其他NDM-1酶相比，在我们的分离物中发现的NDM-1酶序列是高度保守的。此外，分子对接研究表明NDM-1通过氢和锌配位键鉴定与不同的羧酮结合。总之，我们介绍了从不同医院分离的NDM-1产生的K.肺炎群菌株的分子表征，也为分子复合物NDM-1 / CarbapeNem抗生素提供原子水平见解。

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