为了探讨新疆胀果甘草总黄酮(general flavonoids,GFs)对宫颈癌细胞的细胞毒活性,自制备胀果甘草GFs,通过离子阱喷雾式质谱技术分析,建立GFs组分的指纹图谱;并且对SiHa宫颈癌细胞进行GFs药物干预,应用MTT法和流式细胞技术分别鉴定细胞活力和细胞凋亡率.获得了新疆胀果甘草GFs组分的特征性指纹图谱,提取物中GFs含量可达35.40%;发现在0～500 μg/mL GFs浓度范围内,GFs药物干预引起细胞活力梯度性下降,其MTT法检出的最低值为12%;在0～1000 μg/mLGFs浓度范围内,GFs诱导的细胞凋亡率呈现梯度性上升趋势,其流式细胞仪检出的最高值为78%.结果表明新疆胀果甘草GFs具有较强的细胞毒活性,能够抑制宫颈癌细胞生长与活力,并诱导细胞凋亡.%To investigate the cytotoxic activity of general flavonoids (GFs) from Xinjiang Glycyrrhiza infiata Bat,on cervical carcinoma cells.GFs was prepared from Glycyrrhiza infiata, and the finger print map was characterized by Electro Spray Mass Spectrometry; SiHa cervical carcinoma cells were treated with GFs, and the cell viability and cellular apoptosis were determined by MTT method and flow cytometry,respectively.We discovered that GFs from Xinjiang Glycyrrhiza infiata had special finger print characteristics;the content of flavonoids in the extract was 35.40％ ;in the dose range of 0-500 μg/mL GFs, the cell viability dropped gradually with the GFs treatment, and the lowest level by MTT detection was 12％ ;in the dose range of 0-1000 μg/mL GFs,the ratio of cellular apoptosis induced by GFs increased gradually,and the highest level by flow cytometry detection was up to 78％.GFs from Xinjiang Glycyrrhiza infiata had relatively high cytotoxic activity including the inhibition of cell growth and viability as well as the induction of cellular apoptosis.In conclusion,this work may provide important evidence for further screening and isolation of active ingredients of flavonoids as well as the development of new natural anti-cancer drugs.
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