选用大麻新品种龙大麻一号的下胚轴进行组织培养,对无菌苗的获得激素浓度、激素配比等影响组培效果的主要因素进行探讨,愈伤及不定芽诱导的最佳激素组合为ZT 1.1 mg·L-1和IAA 1.1 mg·L-1.研究初步建立了龙大麻一号的离体培养再生体系,为转基因大麻工作奠定了研究基础.%The hypocotyls of the new cultivar Longdama No.l of Cannabis saliva L, were used as explants for tissue culture in vitro. A regeneration system for culturing was set up through the study on the physiological state of aseptic seedlings, hormone types and concentrations, and other influential fators. The best combination of hormones were ZT 1.1 mg·L-1 and IAA l.lmg·L-1 for the calli and induction of adventitious buds. The building of this regeneration system could lay a foundation for hemp transgenic research.
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