目的 了解广西口岸东南亚旅行人群腹泻病的病原谱及分子流行特征.方法 收集2016年8月至2017年10月广西各口岸送检的80名腹泻病患者肛拭子样本,使用多重PCR方法进行肠道常见病原体检测,并对诺如病毒阳性样本进行扩增测序.结果 本研究中的旅行者腹泻的总阳性率为40%(32/80),其中诺如病毒是最主要的病原12例,其次为肠道腺病毒9例,轮状病毒8例,星状病毒3例.其中诺如病毒主要以GⅡ.4型为主(10例),其次为GI.6型1例,GⅡ、GI混合感染1例,GⅡ.4与2012年澳大利亚Sydney NSW0514株的同源性达到98%.结论 广西口岸东南亚旅行人群的腹泻病原体以诺如病毒为主,GⅡ.4型是广西口岸东南亚国际旅行者主要的流行毒株,同时存在GⅡ、GI病毒混合感染的情况.%Objective To understand the pathogen spectrum and molecular epidemic characteristics of diarrheal disease in southeast Asian travelers at Guangxi port.Methods From August 2016 to October 2017,80 samples of anal swabs from patients with diarrheal diseases sent to various ports in Guangxi were collected,and multiple PCR methods were used to detect common intestinal pathogens.And norovirus positive samples were amplified and sequenced.Results The total positive detection rate of traveler's diarrhea in this study was 40%(32/80),including 12 cases of norovirus,the most important pathogen,followed by 9 cases of intestinal adenovirus,8 cases of rotavirus,and 3 cases of astrovirus.Among them,such as virus mainly G iv(10 cases),followed by GI.6 in 1 case,GⅡ,GI mixed infection in 1 case,GⅡ.4and 2012 Australian Sydney NSW0514 strain homology of 98%.Conclusion Norovirus was that of the main pethogens of traveler's diarrhea in people travelled to Southeast Asian.And the GⅡ.4 was that of the predominant epidemic genotype at Guangxi port,at the same time there is GⅡ and GI mixed infection.
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