目的 探讨蝙蝠蛾被孢菌丝体多糖免疫调节作用的机理.方法 将不同剂量的蝙蝠蛾被孢菌丝体多糖加入体外培养的小鼠淋巴细胞中,观察其对淋巴细胞增殖的影响.另外取CPSM培养的巨噬细胞RAW264.7,观察CPSM对巨噬细胞增殖、NO释放及吞噬中性红功能的影响.结果 CPSM具有促进体外培养的小鼠脾脏淋巴细胞增殖及促进巨噬细胞RAW264.7增殖、释放N0及吞噬功能.结论 本研究为冬虫夏草增强免疫机能作用提供了依据.%Objective To elucidate immunomodulatory mechanism of polysaccharide from Mortierella hepiali ( CPSM ) . Methods The lymphocyte and macrophages was treated with different concentrations of CPSM. The stimulation effect of CPSM on normal mice lymphocyte proliferation was assayed by MTT method.The phagocytic ability of macrophage RAW264.7 was evaluated with neutral red dye and the stimulation effect of CPSM on NO production by macrophage RAW264.7 was measured by Griess reagents. Result Pharmacological experiments showed that CPSM can promote normal mice lymphocyte proliferation and the ability of macrophage RAW264.7 in phagocytizing neutral red, and raising the level of NO production. conclusion: CPSM can promote the fuction of murine immunocytes.
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