Based on the relevant properties of EST-SSR gene function, and the high polymorphism and potential regulatory function of Genomic-SSR, the polymorphism effect and transferability of EST-SSR and genomic-SSR of eucalypt trees was comparatively studied in this article. A genomic DNA pooling method was developed to rapidly screen eucalypt SSR primers. A total of 340 pairs of eucalypt SSR primers were screened from 395 pairs designed by optimizing SSR-PCR system, and the screening ratio was 86. 06% . Genomic-SSR and EST-SSR screened 204 pairs (ratio 83. 26% ) and 136 pairs (ratio 90. 67% ), respectively. The result indicates that these obtained SSR primers have good polymorphism and transferability in eucalypt trees, and are suitable for SSR marker analysis of various eucalypt species. Compared with the conventional single-template screening method, the first round of screening primers by genomic DNA pooling technology has reduced more than 82% of workload and reagent consumption. The findings lay a foundation for all-aspect, fast and accurate application of SSR marker technology in eucalypt trees.
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