目的：观察右美托咪啶对脂多糖致伤大鼠肾小管上皮细胞 TLR4表达影响，探讨其可能机制。方法：将大鼠肾小管上皮细胞复苏后培养，加入10ng／ml 脂多糖（LPS），均经培养后分为3组，A 组为空白对照组，B 组为对照组，加入右美托咪定2．5μg ，C 组为实验组，先加入阿替美唑10μg 后30min 加入右美托咪定2．5μg ，培养12h ，免疫组化法检测 TLR4蛋白表达及 TLR4 mRNA水平，并测定细胞凋亡率。结果：B 组应用右美托咪定后，TLR4水平为（45．1±13．5）×103均低于A 组、B 组（tBA ＝6．549，tBC ＝5．660，P ＜0．05）；B 组 TLR4 mRNA 相对表达值为0．46±0．02，均低于 A 组、C 组（tBA ＝27．599，tBC ＝24．749，P＜0．05）。 B 组使用右美托咪定后，细胞凋亡率为（34．6±7．6）％，均明显低于 A 组、C 组（ P ＜0．05）。结论：右美托咪啶可下调脂多糖致伤大鼠肾小管上皮细胞 TLR4表达，且右美托咪啶对 TLR4的调节与α2AR 被激动相关。%Objective :To observe the effect of dexmedetomidine for Toll receptor 4 in renal tubular epi‐thelial cells on rat injured by lipopolysaccharide ,and explore the possible mechanism .Methods :Rat renal tubular epi‐thelial cells and recovery ,training ,then they were added 10 ng/ml lipopolysaccharide(LPS)to culture and divided in‐to A group(blank control group) ,group B(control group) was added 2 .5 μg dexmedetomidine ,group C (experi‐mental group)was joined the 10μg atipamezole ,2 .5 μg dexmedetomidine after 30min ,developing 12h ,then took im‐munohistochemistry to measure expression of TLR4 protein and TLR4 mRNA ,and determine the apoptosis rate of them .Results :Toll receptor 4 level was (45 .1 ± 13 .5) × 103 in group B adopted dexmedetomidine and was lower than those of A and B group ,(tBA = 6 .549 ,tBC = 5 .660 ,P< 0 .05) ,B group’ TLR4mRNA was (0 .46 ± 0 .02)and lower than those of A and C group ,( tBA = 27 .599 ,tBC = 24 .749 ,P< 0 .05) .The apoptosis rate of group B was (34 . 6 ± 7 .6)% and significantly lower than that in A and C group(P < 0 .05) .Conclusion :Dexmedetomidine can down Toll receptor 4 expression in renal tubular epithelial cells of rat injured by lipopolysaccharide ,and the regulation for TLR4 of dexmedetomidine is related with α 2AR excited .
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