Objective: To explore the application of PCR technology in the clinical treatment of Helico bacter pylori associated gastric ulcer. Methods: A retrospective analysis was made on 64 patients with helicobacter pylori associated gastric ulcer: ①different parts of the PCR-positive rate before and after treatment; ②the PCR anal ysis of different gastric ulcer healing and pain levels before and after treatment. Results: The PCR positive rate of different parts after treatment decreased, and ulcer healing and pain situation improved with significant differences of different parts. The PCR positive of gastric biopsies and gastric juice still can be found in bacterial culture negative samples at 8 th week after treatment, while there are bacterial culture positive samples in gastric juice negative. Con clusion: The PCR positive rate can reflect the situation of treatment and recovery, but there are significant differ ences between different pats; Compared with bacterial culture, the positive rate and accuracy of gastric biopsy is high, worth being used to detect the helicobacter pylori.%目的:探讨PCR技术在幽门螺杆菌相关胃溃疡临床治疗中的应用.方法:采用PCR技术对64例幽门螺杆菌相关胃溃疡患者进行检测,对比治疗前后不同部位的PCR阳性率和治疗后不同胃溃疡愈合及疼痛水平.结果:治疗后患者不同部位的PCR阳性率均降低,溃疡愈合及疼痛水平均改善,且不同部位之间的阳性率有显著性差异(P＜0.05);治疗8周时,细菌培养阴性患者中仍可检测到胃活检和胃液PCR阳性,但后者阴性患者中仍可检测到细菌培养阳性.结论:PCR检测阳性率可反应患者治疗情况及恢复情况,但不同部位的检出率不同,与细菌培养相比,胃活检具有较高的阳性率和准确率.
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