Objective To study the effects of silencing ADAM17 on the proliferation,migration,and invasion of human thyroid papillary carcinoma K1 cells. Methods K1 cells were divided into the control group,nonsense shRNA group,and ADAM17-shRNA group. Nonsense-shRNA and ADAM17-shRNA were transfected into K1 cells of the nonsense shRNA group and ADAM17-shRNA group by lentiviral transfection technology. The same amount of PBS was added into the control group. The expression levels of ADAM17 were detected by RT-PCR and Western blotting. MTT assay and flow cytometry were used to assess the cell proliferation ability and the cell cycle. The cell migration and invasion abilities were detected by Transwell migration and invasion assay,respectively. Results Compared with the control group and nonsenseshRNA group,the mRNA and protein expression levels of ADAM17 significantly decreased in the ADAM17-shRNA group(all P < 0.05); the optical density (OD value) of the ADAM17-shRNA group at 24,48,and 72 h was significantly lower that that of the control group and nonsense-shRNA group (all P < 0.05); in the ADAM17-shRNA group,the cells arrested in G0/G1 phase,and significant difference was found between the ADAM17-shRNA group and the control group and nonsense-shRNA group (all P < 0.05); compared with the control group and nonsense-shRNA group,the transmembrane cells decreased sharply in the ADAM17-shRNA group (all P < 0.05). Conclusion Silencing ADAM17 can inhibit the proliferation, migration,and invasion of K1 cells.%目的 研究靶向沉默解聚素-金属蛋白酶-17(ADAM17)基因对人甲状腺乳头状癌细胞增殖、迁移及侵袭的影响.方法 取人甲状腺乳头状癌K1细胞,分为空白对照组、无意义序列组、转染组,转染组、无意义序列组分别用整合有ADAM17-shRNA、无意义序列-shRNA的慢病毒转染,空白对照组加入等量PBS液.采用RT-PCR方法 检测各组ADAM17 mRNA相对表达量,Western blotting法测定ADAM17蛋白相对表达量,MTT法评估细胞增殖能力,流式细胞仪测定细胞周期,Transwell迁移和侵袭实验观察细胞的迁移及侵袭能力.结果 与空白对照组、无意义序列组对比,转染组ADAM17 mRNA及蛋白的相对表达量均降低(P均<0.05);转染组在培养24、48、72 h时的细胞增殖OD值均低于空白对照组、无意义序列组(P均<0.05);与空白对照组和无意义序列组比较,转染组细胞周期中G0/G1期细胞所占百分比升高(P均<0.05);与空白对照组、无意义序列组相比,转染组Transwell迁移及侵袭实验穿膜细胞数目减少(P均<0.05).结论 靶向沉默ADAM17基因可抑制K1细胞增殖、迁移及侵袭.
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