Objective To investigate the therapeutic effect and mechanism of resveratrol (Res) on human T lymphatic leukemia cells (Hut78) .Methods Human T lymphocytic leukemia (Hut78) cells were selected as the target cells, which were treated with 6.25, 12.5, 25, 50, 100 and 200μmol/L Res, respectively.CCK-8 assay was used to detect the cell inhibition rate of Hut78 cells which were treated with 100μmol/L Res for 12, 24 and 48 h, respectively.Flow cytometry was used to detect the apoptosis of Hut78 cells which were respectively treated with 100 and 200μmol/L Res for 12 and 24 h.Western blotting was used to detect the expression of C-myc protein in Hut78 cells which were respectively treated with 100 and 200μmol/L Res for 48 h. Results Resveratrol whose dose was greater than 6.25μmol/L significantly inhibited the proliferation of Hut78 cells, and was time-and dose-dependent (all P<0.01).Res induced the apoptosis of Hut 78 cells, which was dose-dependent (all P<0.01). Res own-regulated the expression of C-myc protein which was in a dose-dependent manner (all P<0.01).Conclusion Res-veratrol has significant anti-leukemia effect on human T lymphatic leukemia, and its mechanism may be associated with the down-regulated expression of C-myc protein.%目的：探讨白藜芦醇对人急性T淋巴细胞白血病的体外治疗作用及其机制。方法以人T淋巴细胞白血病细胞（Hut78）作为白藜芦醇的作用靶细胞，分别用6．25、12．5、25、50、100、200μmol／L白藜芦醇处理Hut78细胞24 h，用100μmol／L白藜芦醇处理Hut78细胞12、24、48 h，采用CCK-8法测算细胞抑制率。经100、200μmol／L白藜芦醇分别处理Hut78细胞12、24 h后，用流式细胞术分析Hut78细胞的凋亡情况。 Hut78细胞经100、200μmol／L白藜芦醇处理48 h，采用Western blotting法检测细胞中C-myc蛋白的表达。结果白藜芦醇在大于6．25μmol／L时显著抑制Hut78细胞的增殖，并呈剂量-时间依赖性（P均＜0．01）。白藜芦醇可诱导Hut78细胞凋亡，随浓度增加凋亡细胞增多（P均＜0．01）。白藜芦醇可呈剂量依赖性下调C-myc蛋白表达（P均＜0．01）。结论白藜芦醇有明显的体外抗人急性T淋巴细胞白血病效应，其作用机制可能与下调C-myc蛋白表达有关。
展开▼
