目的：观察脓毒症大鼠肝脏组织中免疫相关基因表达的改变。方法将120只Wistar大鼠按随机数字表法分为假手术组30只、模型组90只，模型组根据选模后不同时间点（24、48、72 h）随机分为3个亚组各30只。通过盲肠结扎穿孔术（ CLP）复制脓毒症大鼠模型，应用含有28000个大鼠基因cDNA克隆的基因芯片，检测脓毒症大鼠肝组织在CLP后各个时点的免疫相关基因表达变化并筛选出差异基因。结果造模24 h，筛选出差异基因671条，免疫相关基因97条（表达上调48条、下调49条）；造模48 h，筛选出差异基因133条，免疫相关基因36条（表达上调13条、下调23条）；造模72 h，筛选出差异基因204条，免疫相关基因33条（表达上调18条、下调15条）。3个时间点重复基因共58条，免疫相关基因15条（表达上调4条、下调10条、先上调后下调1条）。结论脓毒症大鼠肝组织中，一系列与细胞防御及免疫功能相关的基因表达出现显著改变，它们可能在不同程度上参与了脓毒症大鼠肝损伤的病理生理过程。%Objective To observe the change of immune related gene-expression profiling in rats with sepsis.Meth-ods 120 male wistar rats were randomly divided into sham operation group ( n=30 ) and model group ( n=90 ) .The model group according to different time points (24 h, 48 h and 72 h) were randomly divided into 3 groups ( each subgroup n=30).The rats of model group received cecal ligature and puncture (CLP) operation.Gene chips containing 28 000 rot-genes cDNA were used to test gene-expression in liver of septic rats to select the genes with different expressions with soft-ware.Results Model group had 671 differential expression genes with 97 immune related genes at 24 h;133 differential expression genes with 36 immune related genes at 48 h;204 differential expression genes with 33 immune related genes at 72 h.Three time points in model group and sham operation group repeated gene were 58, immune related gene 15.Con-clusion A series of cell defense and immune function genes change significantly in liver tissue of septic rats, which may be involved in the pathophysiology of sepsis induced hepatic injury in different extent.
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