Objective To observe the status of promoter methylation of Ras association domain family protein 1 A (RASSFlA)gene in osteosarcoma tissue,and explore the clinical significance. Method Methylation-specific PCR was a-dopted to investigate the promoter methylation of RASSF1A gene in 30 pairs of osteosarcoma and corresponding non-tumor tissues. Results The frequency of promoter hypermethylation of RASSF1A showed 46.7% and 13.3% in tumor and core-sponding non-tumor tissues respectively, and there was significant diference between them (P <0. 05). Promoter methylation of RASSF1A in osteosarcoma was related to the gender, age and Alkaline phosphatase ( ALP). Conclusion RASSF1A methylation is a frequent event in osteosarcoma and probably participates in the progression of osteosarcoma.%目的 观察骨肉瘤组织中Ras相关区域家族1A(RASSF1A)基因启动子区域的甲基化状态,并探讨其临床意义.方法 应用甲基化特异性PCR(MSP)技术检测30例骨肉瘤组织及瘤旁组织中RASSF1A基因启动子区域的甲基化状态.结果 骨肉瘤组织中RASSF1A基因启动子区域发生甲基化14例(46.7％),瘤旁组织中4例(13.3％),两者比较,P＜0.05.RASSF1A基因启动子区甲基化与骨肉瘤患者的性别、年龄及血清碱性磷酸酶水平有关(P均＜0.05).结论 骨肉瘤组织中RASSF1A基因启动子区甲基化发生率高,其可能在骨肉瘤的发生发展中发挥重要作用.
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