Objective To investigate the effect of survivin ASODN transfection on the growth, and the expression of survivin, PTEN and CEACAM-1 of PANC cells transplant tumor of nude mice, to sutdy the mechanism. Methods All 28 nude mouse models bearing human pancreatic carcinoma xenograft were constructed and randomly assigned to control group, SODN group, ASODN group and lipofectin group. Each group has 7 nude mice and PBS, SODN, ASODN, lipofectin + DMEM were injected in to the tumor or around the tumor, respectively. Two days after the end of treatment, tumors were fully resected from each mouse and measured for tumor weight and inhibitory rate of tumor growth. Immunohistochemical and TUNEL method was used to evaluate the variations of pathological morphology, survivin, PTEN and CEACAM-1 protein. The survivin mRNA, PTEN mRNA and CEACAM-1 mRNA was measured by RT-PCR respectively. Results Compared with the other three groups, group ASODN tumor growth inhibition rate, growth index was low, and apoptosis index was rasied (all P < 0.05 ) , the expression of survivin and CEACAM-1 was decreased and PTEN was increased in tumor tissue (all P <0.05). Conclusion Survivin ASODN can effectively suppress human pancreatic cancer cells growth in subcutaneous tumor of nude mice and accelerate the induction of apoptosis of tumor cells, the mechanism may be related to the inhibition of survivin and CEACAM-1 expression, the promotion of PTEN expression.%目的 观察survivin反义寡核苷酸(ASODN)转染对人胰腺癌PANC细胞裸鼠移植瘤生长及瘤组织中survivin、PTEN、CEACAM-1表达的影响,并探讨其作用机制.方法 构建28只人胰腺癌PANC细胞裸鼠移植瘤模型,随机分为对照组、正义寡核苷酸(SODN)组、ASODN组、脂质体组,各7只,分别于瘤周及瘤体注射50 μL的PBS、SODN、ASODN、脂质体+无血清DMEM.注射结束后2d,切取瘤体,测算肿瘤生长抑制率和生长指数,免疫组化法检测瘤组织中的survivin、PTEN、CEACAM-1蛋白,TUNEL染色镜下观察细胞凋亡情况,半定量RT-PCR检测瘤组织中的survivin mRNA、PTEN mRNA、CEACAM-1 mRNA.结果 与其他三组相比,ASODN组肿瘤生长抑制率高、生长指数低、细胞凋亡指数高(P均＜0.05),其瘤组织中survivin和CEACAM-1表达下降、PTEN表达上升(P 均＜0.05).结论 survivin ASODN可有效抑制人胰腺癌裸鼠移植瘤的生长并诱导肿瘤细胞凋亡,其机制可能与抑制survivin和CEACAM-1的表达、促进PTEN的表达有关.
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