Objective To prepare zinc-containing micro-nano patterned titanium surface and preliminarily study its effects on osteoblast behavior and antibacterial properties.Methods Hydrofluoric acid-etching,alkali-and heat-treatment,and hydrothermal treatment with a ZnSO4 solution were performed in sequence to synthesize two zinc-containing micro-nano topological structures on pure titanium surfaces(15-and 60-minute treatment groups).The SLA titanium surface was used as the control group,and two zinc-containing micro-nano patterned titanium surfaces were used as the experiment groups.The micro-topography and surface elemental compositions of different titanium surfaces were observed and analyzed.MC3T3-E1 cells were seeded in the samples of three groups to evaluate the effects of different titanium surfaces on osteoblast behavior.Staphylococcus aureus were seeded in the samples of three groups to investigate antibacterial properties of different titanium surfaces.Results Two micro-nano topological structures,where uniform networks and contained trace zinc appeared,were prepared on pure titanium surfaces.Compared with the control SLA titanium surface,the titanium surfaces modified by two zinc-containing micro-nano topological structures significantly up-regulated the spreading,proliferation and expressions of osteogenic related proteins of MC3T3-E1 cells and had prominent antibacterial properties,especially the 15-minute treatment group.Conclusion The zinc-containing micro-nano topological titanium surface can promote the adhesion,proliferation and differentiation of osteoblasts and has satisfactory antibacterial property.%目的 制备载锌微纳米拓扑结构修饰的钛表面并探究其对成骨细胞行为的影响及其抗菌性能.方法 通过氢氟酸酸蚀、碱热处理、硫酸锌水浴热处理,在纯钛表面制备两种载锌微纳米拓扑结构(15、60 min处理组).以大颗粒喷砂酸蚀处理(SLA)钛表面为对照组、两种载锌微纳米拓扑结构修饰的钛表面为实验组,观察分析不同钛表面的微形貌和表面元素组成;将MC3T3-E1成骨细胞接种于各组试件表面,研究不同钛表面对成骨细胞行为的影响;将金黄色葡萄球菌接种于各组试件表面,研究不同钛表面的抗菌性能.结果 在纯钛表面制备形成两种形貌均一的微纳米网络拓扑结构,均含有微量锌元素.较之SLA钛表面,两种载锌微纳米拓扑结构修饰的钛表面能明显上调MC3T3-E1成骨细胞的粘附铺展、增殖和成骨相关蛋白表达,并具有显著的抗菌性能,15 min处理组性能更优.结论 载锌微纳米拓扑结构修饰的钛表面能有效促进成骨细胞的粘附、增殖和分化,且具有良好的抗菌性能.
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