目的：探讨下调蛋白磷酸酯酶2A(PP2A)活性对胎鼠海马神经元轴突生成的影响。方法选取体外培养原代海马神经元为研究模型；采用 PP2A 抑制剂 OA 下调 PP2A 活性，最 后 采用 荧 光 双 标 检 测PP2A 对神经元轴突的影响。结果对照组海马神经元培养至72h 时神经元轴突已经形成，而 OA 处理后的神经元轴突生长明显受到抑制；OA 处理后的神经元轴突长度分别为对照组(DMSO)的47%，单个神经元的平均轴突数目也从正常1.1euron 下降到0.6euron。结论下调 PP2A 活性能抑制原代海马神经元轴突生成，提示 PP2A 在海马神经元轴突生成中起重要作用。%Objective To explore the effect of downregulation of PP2A on fetal rat hippocampal neuro-nal axon outgrowth.Methods Primary hippocampal neuron systems was chosed as the model,the Okadaic acid (OA)was used to inhibit the PP2A activity.Cells were measured by double immunofluorescence to detect the ax on outgrowth.Results Hippocampal neurons were treated with 10 nmol/L OA for 48 h when they were cultured for 24 h.Then cells were measured the alterations of axons by using double immunofluorescence.Da-ta showed that Axon-dendrite polarity was established after culturing for 72 h in DMSO treated groups under the present conditions.However,the formation of axons were inhibited significantly when treated with OA. Further statistical analysis data showed that OA-treated axon average length were only 47% of control group (DMSO),and the average number of axons of single neuron also dropped from the normal 1 .1euron to 0.6euron.Conclusions Downregulation of PP2A could inhibit neuronal axon outgrowth,which indicated that PP2A plays an important role in the hippocampus neuronal axon outgrowth.
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