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Acutely Exposed Hippocampal Neurons: A Preparation for Patch Clamping Neuronsfrom Adult Hippocampal Slices

机译:急性暴露的海马神经元:成人海马切片修补钳夹神经元的准备

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Pharmacological agents known to modulate LTP at the mossy fiber-to-CA3 pyramidalneuron synapse were tested for their effects on the activity of single voltage-gated calcium channels in adult CA3 pyramidal neurons. Single-channel current recordings of three types of voltage-gated calcium channels were made from acutely-exposed CA3 pyramidal neurons of the adult guinea pig hippocampus. The beta-adrenergic agonist isoproterenol which is known to enhance LTD, increased the activity of the two high-threshold calcium channels (N and L), with no striking effect on the low-threshold (T) channel.

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