Objective To investigate the effect of Corilagin on expression of inflammatory factor and neuronal apoptosis in Herpes simplex encephalitis (HSE). Methods The herpes simplex virus-1(HSV-1) infected BV2 cells model was built, and then randomly divided into five groups; control group, HSV-linfected group, HSV-1 infected + Corilagin group, HSV-linfected + Dexanethasone group and HSV-linfected + Astragalus polysaccharide group. The contents of IL-1b and TNP-α secreted by microglia was detected by ELISA in each group, which revealed the inflammation level. At the same time, HSV-1 was used to infect rats to build HSE model, then the rats were divided into the same group as above, the TUNEL method was applied to measure the degree of neuronal apoptosis in each group. Results After HSV-1 infection, compared with HSV-1 infected BV2 cells group, both HSV-1 infected + corilagin group and HSV-1 infected + Dexanethasone group significantly decreased the expression of IL-1b and TNP-α(P＜0. 01) in HSV-1 infected BV2 cells model . Also, compared with HSV-1 infected rats group, both groups obviously decreased the degree of neuronal apoptosis in HSV-1 infected rats. Conclusions Corilagin may play an important role in protecting brain by reducing the expression of IL-1b-, TNF-α and level of neuronal apoptosis in HSE.%目的 探讨柯里拉京对单纯疱疹病毒型脑炎(HSE)炎性因子分泌和神经元凋亡的影响.方法 建立HSV-1接种BV2细胞模型,随机分为正常组、单纯疱疹病毒1型(HSV-1)感染组、柯里拉京干预组、地塞米松干预组、黄芪多糖干预组,采用ELISA法检测各组炎性因子白细胞介素1β(IL-1β)、肿瘤坏死因子(TNF-a)的表达水平;25只Balb/c小鼠按上述分组分成五组,除正常组外其它各组应用HSV-1感染小鼠建立病毒性脑炎模型,治疗4d后采用TUNEL法观察各组小鼠神经元凋亡的情况.结果 与病毒感染组比较,柯里拉京和地塞米松干预组HSV-1感染后IL-1b和TNF-a的分泌(P<0.01)和神经元凋亡数(P<0.01)均明显减少.结论 柯里拉京可能通过减少HSV-1感染后IL-1b和TNF-a的分泌,并同时抑制神经元的凋亡来发挥其脑保护作用.
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