The rapid development of DNA barcoding technology has greatly promoted the work of plant identification. With the increase of identification work and newly discovered sequences, the technology of ITS2 sequence has become a widely used identification at present. In order to provide certain theoretical reference of plant identification, this study introduced the ITS2 sequence identification process and analyzed the characteristics and problems occurred in the process, based on the characteristics of ITS2 sequence.%DNA条形码技术的迅速发展极大地推动了植物的鉴定工作，随着鉴定工作的不断进行和新序列的不断发现，利用ITS2序列进行鉴定已成为目前较为广泛使用的鉴定技术。本文根据ITS2序列的特点和性质，介绍ITS2序列鉴定的一般过程，并分析其特点和存在问题，以期为植物鉴定方面的研究提供参考。
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