Objective To study the influence of the quorum sensing inhibitor brominated furanone on Porphyromo- nas gingivalis (P^ingivalis) biofilm formation. Methods Doubling dilution method was used to determine the minimal inhibition concentration (MIC) of brominated furanone on P.gingivalis. Absolute ethyl alcohol added in P.gingivalis bacterial suspension was used as the negative control, while P.gingivalis bacterial suspension as blank control. The influences of 1/4MIC, 1/2MIC, MIC, 2MIC of brominated furanone on P.gingivalis biofilm formation were studied by the optical density determination and scanning electron microscope (SEM). Results Four groups of brominated furanone with different concentrations were shown to inhibit Pgingivalis biofilm formation. With the increased concentration of brominated furanone, optical density of P.gingivalis suspension decreased. The biofilm structures of 1/4MIC group, 1/2MIC group and MIC group were loose. Only scattered Pgingivalis cells but no biofilm structure was seen in 2MIC group. Conclusion Brominated furanone could inhibit P.gingivalis biofilm formation without the influence on bacterial growth. The future application of this chemical compound may provide a new possibility for the antimicrobial treatment of periodontal disease.%目的 研究群体感应抑制剂溴化呋喃酮对牙龈卟啉单胞菌生物膜形成的影响.方法 采用二倍稀释法,确定溴化呋喃酮对牙龈卟啉单胞菌的最低抑菌浓度(MIC).含无水乙醇的牙龈卟啉单胞菌菌悬液作为阴性对照组,牙龈卟啉单胞菌菌悬液为空白对照组,通过光密度值测定和扫描电镜观察,研究1/4M1C、1/2MIC、MIC、2MIC等不同浓度溴化呋喃酮对牙龈卟啉单胞菌生物膜形成的影响.结果 不同实验浓度的溴化呋喃酮均可抑制牙龈卟啉单胞菌生物膜形成.随着溴化呋喃酮浓度增加,牙龈卟啉单胞菌菌悬液的光密度值逐渐减小.1/4MIC组、1/2MIC组和MIC组形成的生物膜结构疏松,2MIC组仅见散在的牙龈卟啉单胞菌菌体,未见生物膜结构形成.结论 溴化呋喃酮在不影响细菌生长的情况下,能抑制牙龈卟啉单胞菌生物膜形成.溴化呋喃酮的应用可能为牙周病的抗菌治疗提供一条新的途径.
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