[Objective] Cloning and sequence analysis of the 5'UTR intron of the delta 12-oleic acid desaturase gene GhFAD2-1 would lay the foundation for the study of the expression and regulation of GhFAD2-1.[Method]In this study,the 5'UTR sequence of GhFAD2-1 was cloned by 5'RACE technology.And then,5'UTR intron of GhFAD2-1 was cloned in combination with the cotton genome sequence.Cis elements were also analyzed by PLACE and other bioinformatics software.[Result]The GhFAD2-1 5'UTR sequence 77 bp was obtained from Gossypium hirsutum using 5'RACE technique.The result showed that the transcription start site is T based on a combination of 5'UTR and genome sequence analysis.GhFAD2-1 5'UTR contained a full-length 1,103 bp intron sequence in the A genome of cotton.The full-length sequence of GhFAD2-1 5'UTR intron was 1,111 bp in D genome.The cleavage sites of 5'UTR intron were AA-GG, CA-GC, re-spectively.Cis element analysis showed that the intron included some typical function and optical response re -lated elements and elements related to hormone regulation or stress response.[Conclusion]5'UTR intron se-quences of GhFAD2-1 gene were cloned from the A and D genome,respectively.The transcription start site and the cleavage sites of 5'UTR intron were also identified.The results have laid a foundation to further study expression and regulation of GhFAD2-1 at the molecular level.%[目的]对棉花 Δ12-油酸去饱和酶基因GhFAD2-15'UTR区的内含子进行克隆和序列分析,为研究GhF AD2-1的表达调控奠定基础.[方法]利用5'RACE 技术,克隆GhFAD2-1的5'UTR序列,结合棉花基因组序列,克隆G hFAD2-15'UTR内含子,并利用PLACE等生物信息学软件对其顺式作用原件进行分析.[结果]棉花 A、D基因组的G hFAD2-15'UTR中各含一内含子序列,全长分别为1103 bp、1111 bp;GhFAD2-1成熟mRNA的5'UTR为77bp,转录的起点碱基为T;5'UTR内含子两个剪切位点分别AA-GG、CA-GC.该内含子包括一些典型的与光响应相关的作用元件,以及与激素和胁迫因素相关的应答元件等.[结论]克隆获得了棉花A、D基因组的GhFAD2-1基因5'UTR内含子序列;明确其转录的起点碱基及5'UTR内含子的剪切位点,为进一步在分子水平上研究 GhFAD2-1功能及其表达调控规律,为植物的遗传改良奠定了基础.
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