目的 了解本地区甲氧西林耐药溶血葡萄球菌(MRSH)携带耐药基因情况及其流行状况,提供临床治疗及感染控制的依据.方法 耐药基因的检测采用PCR,菌株同源性分型采用随机扩增DNA 多态性(RAPD)技术.结果 65 株MRSH 的mecA、Aac(6')/aph(2'')、aph(3')-Ⅲ、ermB、ermC 和tetM 耐药基因的阳性率分别为100.0%、93.8%、76.9%、7.7%、96.9%和4.6%,未检测出ermA、tetO 和vanA 耐药基因.依照RAPD 检测方法将65 株MRSH 分为3型,其中A 型占83.1%.结论 本地区的MRSH 携带高比例的mecA、Aac(6')/aph(2'')、aph(3')- Ⅲ和ermC 耐药基因,RAPD分析MRSH 的流行株以A 型为主.%Objective To investigate the prevalent strains in methicillin-resistant Staphylococci haemolyticus (MRSH) and drug-resistant related genes in MRSH in the region. Methods Drug-resistant related genes were detected by polymerase chain reaction (PCR) and homologous strains were typed by random amplified polymorphic DNA (RAPD). Results The test of resistant-related genes in MRSH strains showed that the positive rates of resistant genes mecA, Aac (6')/aph (2"), aph (3')-III, ermB, ermC and tetM were 100.0%, 93.8%, 76.9%, 7.7%, 96.9% and 4.6%, respectively; however, the resistant genes of ermA, tetO and vanA were negative. MRSH strains were classified into 3 types by RAPD and type A accounted for 83.1%. Conclusion There are high positive rate of resistant genes mecA, Aac(6')/aph(2"), aph(3')-III and ermC in MRSH and type A strains are the most prevalent strains in the region.
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