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首页> 美国卫生研究院文献>Clinical and Diagnostic Laboratory Immunology >A Live-Attenuated Chimeric Porcine Circovirus Type 2 (PCV2) Vaccine Is Transmitted to Contact Pigs but Is Not Upregulated by Concurrent Infection with Porcine Parvovirus (PPV) and Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Is Efficacious in a PCV2b-PRRSV-PPV Challenge Model
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A Live-Attenuated Chimeric Porcine Circovirus Type 2 (PCV2) Vaccine Is Transmitted to Contact Pigs but Is Not Upregulated by Concurrent Infection with Porcine Parvovirus (PPV) and Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Is Efficacious in a PCV2b-PRRSV-PPV Challenge Model

机译：已将活弱型2型嵌合猪圆环病毒（PCV2）疫苗传播给接触猪但不会因同时感染猪细小病毒（PPV）和猪繁殖与呼吸综合征病毒（PRRSV）而上调并且在PCV2b-PRRSV-中有效PPV挑战模型

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The live chimeric porcine circovirus type 2 (PCV2) vaccine with the capsid gene of the emerging subtype 2b cloned in the genomic backbone of the nonpathogenic PCV1 is attenuated in vivo and induces protective immunity against PCV2. To further determine the safety and efficacy of this experimental vaccine, we tested for evidence of pig-to-pig transmission by commingling nonvaccinated and vaccinated pigs, determined potential upregulation by simultaneous vaccination and infection with porcine parvovirus (PPV) and porcine reproductive and respiratory syndrome virus (PRRSV), and determined vaccine efficacy by challenging pigs 4 weeks after vaccination with PCV2b, PRRSV, and PPV. Forty-six 21-day-old, PCV2-naïve pigs were randomly assigned to one of six groups. Twenty-nine of 46 pigs were challenged with PCV2b, PRRSV, and PPV at day 28, 8/46 remained nonvaccinated and nonchallenged and served as negative controls, and 9/46 remained nonchallenged and served as vaccination controls. All animals were necropsied at day 49. PCV1-PCV2 viremia was detected in nonvaccinated contact pigs commingled with vaccinated pigs, indicating pig-to-pig transmission; however, PCV1-PCV2 DNA levels remained low in all vaccinated and contact pigs regardless of concurrent infection. Finally, vaccination 28 days before challenge resulted in significantly (P < 0.05) decreased amounts of PCV2 in tissues and sera and significantly (P < 0.05) reduced macroscopic and microscopic lesions. The results of this study indicate that the experimental live-attenuated chimeric PCV2 vaccine, although transmissible to contact pigs, remains attenuated in pigs concurrently infected with PRRSV and PPV and induces protective immunity against PCV2b when it is administered 28 days before PCV2 exposure.
机译：在非致病性PCV1的基因组骨架中克隆了新出现的2b亚型衣壳基因的衣原体2型嵌合猪圆环病毒活疫苗在体内减毒，并诱导了针对PCV2的保护性免疫。为了进一步确定这种实验性疫苗的安全性和有效性，我们通过混合未接种疫苗和接种疫苗的猪来测试猪对猪传播的证据，通过同时接种疫苗以及感染猪细小病毒（PPV）和猪生殖与呼吸综合征来确定潜在的上调病毒（PRRSV），并通过在接种PCV2b，PRRSV和PPV疫苗后4周对猪进行攻击来确定疫苗效力。将46只21天大的PCV2天真猪随机分为六组之一。在第28天，有29头猪中有29头受到PCV2b，PRRSV和PPV的攻击，其中8/46仍未接种疫苗且未受到挑战，并作为阴性对照，而9/46仍未受到挑战并用作疫苗接种的对照。在第49天对所有动物进行尸检。在未接种疫苗的接触猪与接种疫苗的猪混合的情况下检测到PCV1-PCV2病毒血症，表明猪到猪之间传播。但是，无论并发感染如何，所有接种疫苗的猪和接触猪的PCV1-PCV2 DNA含量仍然很低。最后，在攻击前28天接种疫苗可显着（P <0.05）减少组织和血清中PCV2的量，并显着（P <0.05）减少宏观和微观病变。这项研究的结果表明，实验性减毒的嵌合PCV2活疫苗虽然可以传播给接触性猪，但在同时感染PRRSV和PPV的猪中仍然减毒，并且在暴露于PCV2前28天给予PCV2b保护性免疫。

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