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首页> 外文期刊>Virus Research: An International Journal of Molecular and Cellular Virology >Expression of antigenic epitopes of porcine reproductive and respiratory syndrome virus (PRRSV) in a modified live-attenuated porcine circovirus type 2 (PCV2) vaccine virus (PCV1-2a) as a potential bivalent vaccine against both PCV2 and PRRSV
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Expression of antigenic epitopes of porcine reproductive and respiratory syndrome virus (PRRSV) in a modified live-attenuated porcine circovirus type 2 (PCV2) vaccine virus (PCV1-2a) as a potential bivalent vaccine against both PCV2 and PRRSV

机译：猪繁殖与呼吸综合征病毒（PRRSV）抗原表位在改良的减毒活猪圆环病毒2型（PCV2）疫苗病毒（PCV1-2a）中的表达作为针对PCV2和PRRSV的潜在二价疫苗

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Co-infection of pigs in the field with porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) is common and poses a major concern in effective control of PCV2 and PRRSV. We previously demonstrated that insertion of foreign epitope tags in the C-terminus of PCV2 ORF2 produced infectious virions that elicited humoral immune responses against both PCV2 capsid and inserted epitope tags. In this study, we aimed to determine whether the non-pathogenic chimeric virus PCV1-2a, which is the basis for the licensed PCV2 vaccine FosteraTM PCV, can express PRRSV antigenic epitopes, thus generating dual immunity as a potential bivalent vaccine against both PCV2 and PPRSV. Four different linear B-cell antigenic epitopes of PRRSV were inserted into the C-terminus of the capsid gene of the PCV1-2a vaccine virus. We showed that insertion of 12 (PRRSV-GP2 epitope II, PRRSV-GP3 epitope I, and PRRSV-GP5 epitope I), and 14 (PRRSV-GP5 epitope IV) amino acid residues did not impair the replication of the resulting PCV1-2a-PRRSVEPI chimeric viruses in vitro. The four chimeric PCV1-2a viruses expressing PRRSV B-cell linear epitopes were successfully rescued and characterized. An immunogenicity study in pigs revealed that two of the four chimeric viruses, PCV1-2a-PRRSV(EPI)GP3IG and PCV1-2a-PRRSVEPI(EPI)GP5IV, elicited neutralizing antibodies against PRRSV VR2385 as well as PCV2 (strains PCV2a, PCV2b, and mPCV2b). The results have important implications for exploring the potential use of PCV1-2a vaccine virus as a live virus vector to develop bivalent MLVs against both PCV2 and PRRSV. (C) 2015 Elsevier B.V. All rights reserved.
机译：猪与猪圆环病毒2型（PCV2）和猪繁殖与呼吸综合症病毒（PRRSV）共同感染猪是常见的，并且在有效控制PCV2和PRRSV方面引起了人们的极大关注。我们先前证明，在PCV2 ORF2的C末端插入外源抗原决定簇标签会产生感染性病毒粒子，引起针对PCV2衣壳和插入的抗原决定簇标签的体液免疫反应。在这项研究中，我们旨在确定非致病性嵌合病毒PCV1-2a（它是许可的PCV2疫苗FosteraTM PCV的基础）是否可以表达PRRSV抗原表位，从而产生针对PCV2和PCV2的潜在双价疫苗双重免疫力PPRSV。将PRRSV的四个不同的线性B细胞抗原表位插入PCV1-2a疫苗病毒衣壳基因的C端。我们表明插入12（PRRSV-GP2表位II，PRRSV-GP3表位I和PRRSV-GP5表位I）和14（PRRSV-GP5表位IV）氨基酸残基不会损害所得PCV1-2a的复制-PRRSVEPI嵌合病毒在体外。表达PRRSV B细胞线性表位的四种嵌合PCV1-2a病毒已成功获救并鉴定。猪的免疫原性研究表明，四种嵌合病毒PCV1-2a-PRRSV（EPI）GP3IG和PCV1-2a-PRRSVEPI（EPI）GP5IV中的两种引发了针对PRRSV VR2385和PCV2的中和抗体（株PCV2a，PCV2b，和mPCV2b）。该结果对探索PCV1-2a疫苗病毒作为活病毒载体开发针对PCV2和PRRSV的二价MLV的潜在意义具有重要意义。 （C）2015 Elsevier B.V.保留所有权利。

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