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首页> 美国卫生研究院文献>Microorganisms >Thermostable Xylanase Production by Geobacillus sp. Strain DUSELR13 and Its Application in Ethanol Production with Lignocellulosic Biomass
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Thermostable Xylanase Production by Geobacillus sp. Strain DUSELR13 and Its Application in Ethanol Production with Lignocellulosic Biomass

机译：Geobacillus sp。生产的热稳定木聚糖酶。 DUSELR13菌株及其在木质纤维素生物质乙醇生产中的应用

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The aim of the current study was to optimize the production of xylanase, and its application for ethanol production using the lignocellulosic biomass. A highly thermostable crude xylanase was obtained from the Geobacillus sp. strain DUSELR13 isolated from the deep biosphere of Homestake gold mine, Lead, SD. Geobacillus sp. strain DUSELR13 produced 6 U/mL of the xylanase with the beechwood xylan. The xylanase production was improved following the optimization studies, with one factor at a time approach, from 6 U/mL to 19.8 U/mL with xylan. The statistical optimization with response surface methodology further increased the production to 31 U/mL. The characterization studies revealed that the crude xylanase complex had an optimum pH of 7.0, with a broad pH range of 5.0–9.0, and an optimum temperature of 75 °C. The ~45 kDa xylanase protein was highly thermostable with t1/2 of 48, 38, and 13 days at 50, 60, and 70 °C, respectively. The xylanase activity increased with the addition of Cu+2, Zn+2, K+, and Fe+2 at 1 mM concentration, and Ca+2, Zn+2, Mg+2, and Na+ at 10 mM concentration. The comparative analysis of the crude xylanase against its commercial counterpart Novozymes Cellic HTec and Dupont, Accellerase XY, showed that it performed better at higher temperature, hydrolyzing 65.4% of the beechwood at 75 °C. The DUSEL R13 showed the mettle to hydrolyze, and utilize the pretreated, and untreated lignocellulosic biomass: prairie cord grass (PCG), and corn stover (CS) as the substrate, and gave a maximum yield of 20.5 U/mL with the untreated PCG. When grown in co-culture with Geobacillus thermoglucosidasius, it produced 3.53 and 3.72 g/L ethanol, respectively with PCG, and CS. With these characteristics the xylanase under study could be an industrial success for the high temperature bioprocesses.
机译：本研究的目的是优化木聚糖酶的生产及其在使用木质纤维素生物质生产乙醇中的应用。高耐热性的粗木聚糖酶得自Geobacillus sp.。从Homestake金矿深处生物圈分离的DUSELR13菌株，SD。芽孢杆菌DUSELR13菌株与山毛榉木聚糖产生了6 U / mL的木聚糖酶。经过优化研究后，木聚糖酶的产量得到了提高，每次使用一个因素时，木聚糖的产量从6 U / mL提高到19.8 U / mL。使用响应面方法进行的统计优化进一步将产量提高到31 U / mL。表征研究表明，粗木聚糖酶复合物的最佳pH为7.0，pH范围为5.0-9.0，最佳温度为75°C。 〜45 kDa的木聚糖酶蛋白在50、60和70°C时具有48、38和13天的t1 / 2高度耐热性。木聚糖酶活性随Cu +2， Zn +2 ，K +，和Fe +2 浓度为1 mM时，Ca +2 ，Zn +2 ，Mg +2 和Na + 浓度为10 mM。对粗木聚糖酶与商用Novozymes Cellic HTec和Dupont Accellerase XY的比较分析表明，它在较高温度下表现更好，在75°C时水解了65.4％的山毛榉木。 DUSEL R13显示出水解的能力，并利用了预处理的和未处理的木质纤维素生物质：草原草（PCG）和玉米秸秆（CS）作为底物，未经处理的PCG的最大产量为20.5 U / mL 。当与热葡糖芽孢杆菌共培养时，它与PCG和CS分别产生3.53和3.72 g / L乙醇。具有这些特征的被研究的木聚糖酶可能是高温生物过程的工业成功。

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