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首页> 美国卫生研究院文献>International Journal of Biological Sciences >Mesoporous bioactive glass-coated 3D printed borosilicate bioactive glass scaffolds for improving repair of bone defects
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Mesoporous bioactive glass-coated 3D printed borosilicate bioactive glass scaffolds for improving repair of bone defects

机译：介孔生物活性玻璃涂层3D打印的硼硅酸盐生物活性玻璃支架可改善骨缺损的修复

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>Background: In the field of tissue engineering, there is currently increasing interest in new biomedical materials with high osteogenic ability and comparable mechanical function to repair bone defects. Three-dimensional (3-D) bioactive borosilicate glass (BG) scaffolds exhibit uniform interconnected macro-pores, high porosity and high compressive strength. In this study, we fabricated 3-D BG scaffolds by the 3D printing technique, then coated the surface of the 3-D BG scaffolds with mesoporous bioactive glass (MBG) (BG-MBG scaffold).>Methods: The biocompatibility of the BG-MBG scaffolds was evaluated by assessing biodegradability, cell proliferation, alkaline phosphatase (ALP) activity and by quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis of osteogenic gene expression with human bone marrow stromal cells (hBMSCs). Moreover, the BG-MBG scaffolds were used to repair rat femoral defects and their performance was evaluated using microcomputed tomography (micro-CT), fluorescence labeling, histological analysis and immunohistochemical (IHC) analysis.>Results: The results showed that the BG-MBG scaffolds possessed ordered nearly 4nm meso-pores and regular macro-pores, as well as good biodegradability, and that they stimulated the proliferation and osteogenic differentiation of hBMSCs. In in vivo studies, the result of micro-CT reconstructed images (BG-9M group, 0.63 ± 0.02 g/cm3 and BG group 0.13 ± 0.02 g/cm3 ) and van Gieson staining (BG-9M groups, 62.67 ± 3.39% and BG group, 12.33 ± 2.58%) showed that the BG-MBG scaffolds could significantly enhance new bone formation in both inner and peripheral scaffolds in defects, in and in without the presence of growth factors or stem cells (P < 0.05).>Conclusions: It is believed from these results that the BG-MBG scaffolds possess excellent osteoinductive and osteogenic properties which will make them appealing candidates for bone defect repair. The novelty of our research is to provide a new material to treat bone defects in clinic.
机译：>背景：在组织工程领域，目前人们对具有高成骨能力和相当的机械功能来修复骨缺损的新型生物医学材料越来越感兴趣。三维（3-D）生物活性硼硅酸盐玻璃（BG）支架表现出均匀的互连大孔，高孔隙率和高抗压强度。在这项研究中，我们通过3D打印技术制造了3-D BG支架，然后用中孔生物活性玻璃（MBG）（BG-MBG支架）涂覆了3-D BG支架的表面。>方法：通过评估生物降解性，细胞增殖，碱性磷酸酶（ALP）活性并通过定量逆转录聚合酶链反应（qRT-PCR）分析人骨髓基质细胞（hBMSCs）的成骨基因表达，评估了BG-MBG支架的生物相容性）。此外，BG-MBG支架用于修复大鼠股骨缺损，并通过微计算机断层扫描（micro-CT），荧光标记，组织学分析和免疫组化（IHC）分析评估了它们的性能。>结果：结果表明，BG-MBG支架具有近4nm的有序介孔和规则的大孔，并且具有良好的生物降解性，并刺激了hBMSCs的增殖和成骨分化。在体内研究中，显微CT重建图像的结果（BG-9M组，0.63±0.02 g / cm 3 和BG组0.13±0.02 g / cm 3 ）和van Gieson染色（BG-9M组，62.67±3.39％和BG组，12.33±2.58％）显示，BG-MBG支架可以显着增强内部和周围支架中的新骨形成，无论是否存在缺陷生长因子或干细胞的存在（P <0.05）。>结论：从这些结果可以相信，BG-MBG支架具有优异的骨诱导和成骨特性，这将使其成为骨缺损的诱人候选物。修理。我们研究的新颖性是为临床提供一种治疗骨缺损的新材料。

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