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首页> 美国卫生研究院文献>Infection and Immunity >Use of restriction endonuclease analysis and ribotyping to study epidemiology of Pasteurella multocida in closed swine herds.
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Use of restriction endonuclease analysis and ribotyping to study epidemiology of Pasteurella multocida in closed swine herds.

机译：使用限制性核酸内切酶分析和核糖分型法研究封闭猪群中多杀性巴氏杆菌的流行病学。

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摘要

One hundred and sixty-four clinical isolates of Pasteurella multocida recovered from two swine herds in Minnesota were characterized by restriction endonuclease analysis (REA) and rRNA gene restriction fragment length patterns. Bacterial DNA was digested with HpaII and electrophoresed in 0.55% agarose. Restriction fragments were transferred by Southern blot to nylon membranes and then hybridized with digoxigenin-dUTP-labeled Escherichia coli rRNA. Four different REA patterns were observed among the 156 serotype A strains isolated from herds A and B. The two most common REA types (1 and 2) represented 92% of the strains analyzed, while REA types 3 and 4 were observed only in lung samples and accounted for 8% of the isolates. Two different ribotypes were observed for these serotype A isolates. Ribotype I consisted of the most common types, 1 and 2, found by DNA fingerprinting. Ribotype II included REA types 3 and 4. Results from both herds suggest that in closed swine populations, a single strain of P. multocida predominates and causes disease. It is concluded that these genomic fingerprinting techniques were highly discriminatory and that capsular serotyping in combination with REA or ribotyping is an appropriate technique for epidemiological studies of P. multocida of swine origin.
机译：从限制性内切酶分析（REA）和rRNA基因限制性片段长度模式对从明尼苏达州的两个猪群中回收的一百六十四株多杀巴斯德氏菌临床分离株进行了表征。用HpaII消化细菌DNA，并在0.55％琼脂糖中电泳。通过Southern印迹将限制片段转移至尼龙膜，然后与洋地黄毒苷-dUTP标记的大肠杆菌rRNA杂交。在从牛群A和牛群B分离的156种血清型A菌株中观察到四种不同的REA模式。两种最常见的REA类型（1和2）代表了所分析菌株的92％，而仅在肺样品中观察到了REA 3和4型。占分离株的8％。对于这些血清型A分离株，观察到两种不同的核糖型。 I型核糖核酸由DNA指纹图谱发现的最常见的1型和2型组成。 II型核糖包括REA 3型和4型。两种猪群的结果都表明，在封闭的猪群中，多杀性疟原虫单株占主导地位并引起疾病。结论是，这些基因组指纹技术具有很高的歧视性，荚膜血清分型与REA或核糖分型相结合是适合猪源多杀性疟原虫流行病学研究的合适技术。

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