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首页> 美国卫生研究院文献>Materials >Cytotoxicity Test of One-Step Self-Etching Bonding Agents by Standardized Dentin Barrier Test Using Polyurethane Discs
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Cytotoxicity Test of One-Step Self-Etching Bonding Agents by Standardized Dentin Barrier Test Using Polyurethane Discs

机译：一步法自腐蚀结合剂的细胞毒性测试通过使用聚氨酯盘的标准化牙本质屏障测试

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This study was performed to standardize a dentin barrier test with the substitute and evaluate the cytotoxicity of one-step self-etching bonding agents. Each of the natural bovine dentin and polyurethane discs were 500-μm thick and were tested using a perfusion device. Following the treatment with 0.05% phenol on the natural bovine disc or three kinds of polyurethane discs—30, 40, and 50 pcf (pounds per cubic foot)—cell viability of L-929 was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and expressed as percentages of non-treated group, respectively. A substitute showing permeability similar to that of bovine dentin was determined based on this result. Cytotoxicity test of bonding agents was performed by the selected substitute, the results of which were expressed as percentages of the control. In addition, SEM images were taken after the tests. The cell viability by 40-pcf polyurethane disc was not statistically different from that by bovine dentin disc (P > 0.05). Futurabond DC resulted in the highest cell viability and Bond force the lowest by the 40-pcf polyurethane disc (P < 0.05). The adhesives on the 40-pcf polyurethane disc changed cellular morphology with different degrees on the SEM images. This standardized test might be useful for assessing the cytotoxicity of dental materials applied to dentin before clinical applications.
机译：进行这项研究的目的是用替代品对牙本质屏障测试进行标准化，并评估一步自蚀刻粘合剂的细胞毒性。天然牛牙本质和聚氨酯盘的厚度均为500μm，并使用灌注设备进行了测试。在天然牛盘或三种聚氨酯盘（30、40和50 pcf（磅/立方英尺））上用0.05％苯酚处理后，通过3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四氮唑（MTT）测定，并分别表示为未处理组的百分比。基于该结果，确定了具有与牛牙本质相似的渗透性的替代物。通过选择的替代物进行粘合剂的细胞毒性测试，其结果表示为对照的百分比。另外，在测试之后拍摄SEM图像。 40-pcf聚氨酯盘的细胞活力与牛牙本质盘的细胞活力无统计学差异（P> 0.05）。 Futurabond DC产生的40-pcf聚氨酯圆盘具有最高的细胞活力，而键合力最低（P <0.05）。 40 pcf聚氨酯圆盘上的粘合剂在SEM图像上以不同程度改变了细胞形态。该标准化测试可能有助于评估在临床应用之前应用于牙本质的牙科材料的细胞毒性。

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