• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Infection and Immunity >Vaccination of Cattle with a CpG Oligodeoxynucleotide-Formulated Mycobacterial Protein Vaccine and Mycobacterium bovis BCG Induces Levels of Protection against Bovine Tuberculosis Superior to Those Induced by Vaccination with BCG Alone
【2h】

Vaccination of Cattle with a CpG Oligodeoxynucleotide-Formulated Mycobacterial Protein Vaccine and Mycobacterium bovis BCG Induces Levels of Protection against Bovine Tuberculosis Superior to Those Induced by Vaccination with BCG Alone

机译：用CpG寡脱氧核苷酸配制的分枝杆菌蛋白疫苗和牛分枝杆菌BCG接种牛所产生的牛结核病防护水平要优于单独接种BCG所诱导的牛结核病水平

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The development of a subunit protein vaccine for bovine tuberculosis which could be used either in combination with Mycobacterium bovis BCG (to improve the efficacy of that vaccine) or alone would offer significant advantages over currently available strategies. A study was conducted with cattle to determine the protective efficacy of a strategy based on concurrent immunization with an M. bovis culture filtrate (CFP) vaccine and BCG compared to vaccination with either vaccine alone. One group of calves (10 animals per group) was vaccinated subcutaneously with CFP formulated with Emulsigen and combined with a CpG oligodeoxynucleotide (ODN). A second group was vaccinated with both the CFP vaccine and BCG injected at adjacent sites (CFP-BCG). One further group was vaccinated subcutaneously with BCG, while another group served as nonvaccinated control animals. Vaccination with CFP-BCG induced levels of antigen-specific gamma interferon (IFN-γ) and interleukin-2 (IL-2) in whole-blood cultures that were higher than those induced by vaccination with BCG alone. The combination of CFP and BCG did not enhance the production of antibodies to M. bovis CFP compared to vaccination with CFP alone. Vaccination with CFP alone led to delayed antigen-specific IFN-γ and IL-2 responses. Vaccination with CFP-BCG induced a high level of protection against an intratracheal challenge with virulent M. bovis, based on a significant enhancement of six pathological and microbiological parameters of protection compared with the nonvaccinated group. In contrast, vaccination with BCG alone induced a significant enhancement of protection in only one parameter, while CFP alone induced no protection. These results suggest that a combination of a CpG ODN-formulated protein vaccine and BCG offers better protection against bovine tuberculosis than does BCG alone.
机译：可以与牛分枝杆菌卡介苗联合使用（提高该疫苗的功效）或单独使用的牛结核病亚单位蛋白疫苗的开发，将比目前可用的策略具有明显的优势。与牛只进行了一项研究，以确定与同时接种牛分枝杆菌培养物滤液（CFP）疫苗和BCG的同时免疫相比，该策略的保护效果。一组犊牛（每组10只动物）用Emulsigen配制的CFP皮下接种，并与CpG寡脱氧核苷酸（ODN）结合。第二组在相邻部位注射了CFP疫苗和BCG（CFP-BCG）。另一组用卡介苗皮下接种，而另一组作为未接种的对照动物。在全血培养物中接种CFP-BCG诱导的抗原特异性γ干扰素（IFN-γ）和白介素2（IL-2）的水平高于单独接种BCG诱导的水平。与单独接种CFP相比，CFP和BCG的结合不会增加针对牛分枝杆菌CFP的抗体的产生。仅用CFP疫苗接种会导致抗原特异性IFN-γ和IL-2反应延迟。与未接种疫苗的组相比，使用CFP-BCG进行的疫苗接种可有效保护抵抗强毒牛分枝杆菌的气管内攻击，这是因为六个保护作用的病理和微生物参数显着提高。相反，仅用BCG接种仅在一个参数上引起保护作用的显着增强，而仅CFP则不引起保护作用。这些结果表明，与单独的BCG相比，CpG ODN配制的蛋白疫苗和BCG的结合对牛结核病的保护作用更好。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号