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首页> 美国卫生研究院文献>Infection and Immunity >Host Contributions to Construction of Three Device-Associated Candida albicans Biofilms
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Host Contributions to Construction of Three Device-Associated Candida albicans Biofilms

机译：主机贡献的三个与设备相关的白色念珠菌生物膜的建设。

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Among the most fascinating virulence attributes of Candida is the ability to transition to a biofilm lifestyle. As a biofilm, Candida cells adhere to a surface, such as a vascular catheter, and become encased in an extracellular matrix. During this mode of growth, Candida resists the normal immune response, often causing devastating disease. Based on scanning electron microscopy images, we hypothesized that host cells and proteins become incorporated into clinical biofilms. As a means to gain an understanding of these host-biofilm interactions, we explored biofilm-associated host components by using microscopy and liquid chromatography-mass spectrometry. Here we characterize the host proteins associated with several in vivo rat Candida albicans biofilms, including those from vascular catheter, denture, and urinary catheter models as well as uninfected devices. A conserved group of 14 host proteins were found to be more abundant during infection at each of the niches. The host proteins were leukocyte and erythrocyte associated and included proteins involved in inflammation, such as C-reactive protein, myeloperoxidase, and alarmin S100-A9. A group of 59 proteins were associated with both infected and uninfected devices, and these included matricellular and inflammatory proteins. In addition, site-specific proteins were identified, such as amylase in association with the denture device. Cellular analysis revealed neutrophils as the predominant leukocytes associating with biofilms. These experiments demonstrate that host cells and proteins are key components of in vivo Candida biofilms, likely with one subset associating with the device and another being recruited by the proliferating biofilm.
机译：念珠菌最引人入胜的毒力特性之一就是能够过渡到生物膜生活方式。作为一种生物膜，念珠菌细胞粘附在诸如血管导管的表面上，并被包裹在细胞外基质中。在这种生长方式下，念珠菌抵抗正常的免疫反应，经常引起破坏性疾病。基于扫描电子显微镜图像，我们假设宿主细胞和蛋白质被整合到临床生物膜中。作为一种了解这些宿主生物膜相互作用的方法，我们通过使用显微镜和液相色谱-质谱技术探索了与生物膜相关的宿主组分。在这里，我们表征与几种体内大鼠白色念珠菌生物膜相关的宿主蛋白，包括来自血管导管，义齿和导尿管模型以及未感染装置的生物膜。发现在每个壁infection的感染过程中，一组保守的14种宿主蛋白​​更为丰富。宿主蛋白与白细胞和红细胞相关，包括与炎症有关的蛋白，例如C反应蛋白，髓过氧化物酶和警报蛋白S100-A9。一组59种蛋白质与感染和未感染的设备相关，其中包括基质细胞和炎性蛋白质。另外，鉴定了位点特异性蛋白质，例如与义齿装置相关的淀粉酶。细胞分析显示嗜中性粒细胞是与生物膜相关的主要白细胞。这些实验表明，宿主细胞和蛋白质是体内念珠菌生物膜的关键组成部分，可能其中一个子集与该装置相关联，而另一个子集则由增殖的生物膜募集。

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