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首页> 美国卫生研究院文献>Infection and Immunity >Vaccination with the Recombinant Major Outer Membrane Protein Elicits Antibodies to the Constant Domains and Induces Cross-Serovar Protection against Intranasal Challenge with Chlamydia trachomatis
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Vaccination with the Recombinant Major Outer Membrane Protein Elicits Antibodies to the Constant Domains and Induces Cross-Serovar Protection against Intranasal Challenge with Chlamydia trachomatis

机译：重组主要外膜蛋白的疫苗接种使抗体具有恒定结构域并诱导针对沙眼衣原体的鼻内攻击的跨血清保护。

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To determine the ability of the major outer membrane protein (MOMP) to elicit cross-serovar protection, groups of mice were immunized by the intramuscular (i.m.) and subcutaneous (s.c.) routes with recombinant MOMP (rMOMP) from Chlamydia trachomatis serovars D (UW-3/Cx), E (Bour), or F (IC-Cal-3) or Chlamydia muridarum strain Nigg II using CpG-1826 and Montanide ISA 720 VG as adjuvants. Negative-control groups were immunized i.m. and s.c. with Neisseria gonorrhoeae recombinant porin B (Ng-rPorB) or i.n. with Eagle's minimal essential medium (MEM-0). Following vaccination, the mice developed antibodies not only against the homologous serovar but also against heterologous serovars. The rMOMP-vaccinated animals also mounted cell-mediated immune responses as assessed by a lymphoproliferative assay. Four weeks after the last immunization, mice were challenged i.n. with 104 inclusion-forming units (IFU) of C. muridarum. The mice were weighed for 10 days and euthanized, and the number of IFU in their lungs was determined. At 10 days postinfection (p.i.), mice immunized with the rMOMP of C. muridarum or C. trachomatis D, E, or F had lost 4%, 6%, 8%, and 8% of their initial body weight, respectively, significantly different from the negative-control groups (Ng-rPorB, 13%; MEM-0, 19%; P < 0.05). The median number of IFU recovered from the lungs of mice immunized with C. muridarum rMOMP was 0.13 × 106. The median number of IFU recovered from mice immunized with rMOMP from serovars D, E, and F were 0.38 × 106, 7.56 × 106, and 11.94 × 106 IFU, respectively. All the rMOMP-immunized animals had significantly less IFU than the Ng-rPorB (40 × 106)- or MEM-0 (70 × 106)-immunized mice (P < 0.05). In conclusion, vaccination with rMOMP can elicit protection against homologous and heterologous Chlamydia serovars.
机译：为了确定主要的外膜蛋白（MOMP）引起交叉血清保护的能力，用沙眼衣原体沙眼衣原体D（UW）的重组MOMP（rMOMP）通过肌内（im）和皮下（sc）途径免疫各组小鼠-3 / Cx），E（布尔）或F（IC-Cal-3）或鼠衣原体Nigg II菌株，使用CpG-1826和Montanide ISA 720 VG作为佐剂。阴性对照组在当天早上免疫。和s.c.用淋病奈瑟氏球菌重组孔蛋白B（Ng-rPorB）或i.n.使用Eagle的基本必需培养基（MEM-0）。接种疫苗后，小鼠不仅产生针对同源血清型的抗体，而且还针对异源血清型产生抗体。如通过淋巴增生测定所评估，rMOMP疫苗接种的动物还具有细胞介导的免疫反应。在最后一次免疫后四周，对小鼠进行i.n攻击。与10.sup> 4 包涵体的C. muridarum。将小鼠称重10天并实施安乐死，并确定其肺中IFU的数量。在感染后第10天（pi），用鼠李糖单胞菌或沙眼衣原体D，E或F的rMOMP免疫的小鼠分别损失了其初始体重的4％，6％，8％和8％。与阴性对照组不同（Ng-rPorB，13％； MEM-0，19％； P <0.05）。从鼠疫衣原体rMOMP免疫的小鼠肺中回收的IFU的中位数为0.13×10 6 。从rMOMP免疫血清D，E和F的小鼠中回收的IFU的中位数为0.38×10 6 ，7.56×10 6 和11.94×10 6 IFU。所有rMOMP免疫的动物的IFU均显着低于Ng-rPorB（40×10 6 ）-或MEM-0（70×10 6 ）免疫的小鼠（ P <0.05）。总之，rMOMP疫苗接种可引起针对同源和异源衣原体血清型的保护。

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