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The Quantitative Analysis of bFGF and VEGF byELISA in Human Meningiomas

机译:bFGF和VEGF的定量分析。人类脑膜瘤的ELISA

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摘要

The quantitative analysis of VEGF using ELISA in various subtypes of grade I meningiomas reported higher VEGF contents in meningothelial (2.38 ± 0.62 pg/μg protein, n = 7), transitional (1.08 ± 0.21 pg/μg protein, n = 13), and microcystic meningiomas (1.98 ± 0.87 pg/μg protein, n = 5) as compared with fibrous ones (0.36 ± 0.09 pg/μg protein, n = 5). In contrast to VEGF, no difference in the concentrations of bFGF was detected. VEGF levels did not correlate with meningioma grade (1.47 ± 0.23 pg/μg versus 2.29 ± 0.58 pg/μg for 32 and 16 grade I and II, resp), vascularisation (1.53 ± 0.41 pg/μg versus 1.96 ± 0.28 pg/μg for 24 low and 24 high vascularisated tumours, resp), and brain invasion (2.32 ± 0.59 pg/μg versus 1.46 ± 0.27 pg/μg for 7 and 41 patients with and without invasion, resp). The ELISA procedure is, thus, an interesting tool to ensure VEGF and bFGF levels in meningiomas and to test putative correlations with clinical parameters. It is, thus, tempting to speculate that ELISA would also be valuable for the quantitative analysis of other angiogenic growth factors and cytokines in intracranial tumours.
机译:使用ELISA对Ⅰ类脑膜瘤亚型的亚型进行的VEGF定量分析显示,脑膜内皮中的VEGF含量较高(2.38±0.62 pg /μg蛋白,n = 7),过渡型(1.08±0.21 pg /μg蛋白,n = 13),并且微囊性脑膜瘤(1.98±0.87 pg /μg蛋白质,n = 5),而纤维性脑膜瘤(0.36±0.09 pg /μg蛋白质,n = 5)。与VEGF相反,未检测到bFGF浓度的差异。 VEGF水平与脑膜瘤​​级别无关(分别为32和16级I和II,分别为1.47±0.23 pg /μg和2.29±0.58 pg /μg,而血管形成)(1.53±0.41 pg /μg对1.96±0.28 pg /μg 24例低和24例高血管形成的肿瘤,分别)和脑浸润(分别为7和41例有无浸润的患者,分别为2.32±0.59 pg /μg和1.46±0.27 pg /μg)。因此,ELISA程序是确保脑膜瘤中VEGF和bFGF水平并测试与临床参数相关性的有趣工具。因此,很容易推测ELISA对于颅内肿瘤中其他血管生成生长因子和细胞因子的定量分析也很有价值。

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