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The Quantitative Analysis of bFGF and VEGF by ELISA in Human Meningiomas

机译:ELISA法对人脑膜瘤中bFGF和VEGF的定量分析

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The quantitative analysis of VEGF using ELISA in various subtypes of grade I meningiomas reported higher VEGF contents in meningothelial (2.38±0.62?pg/μgprotein,n=7), transitional (1.08±0.21?pg/μgprotein,n=13), and microcystic meningiomas (1.98±0.87?pg/μgprotein,n=5) as compared with fibrous ones (0.36±0.09?pg/μgprotein,n=5). In contrast to VEGF, no difference in the concentrations of bFGF was detected. VEGF levels did not correlate with meningioma grade (1.47±0.23?pg/μgversus2.29±0.58?pg/μgfor 32 and 16 grade I and II, resp), vascularisation (1.53±0.41?pg/μgversus1.96±0.28?pg/μgfor 24 low and 24 high vascularisated tumours, resp), and brain invasion (2.32±0.59?pg/μgversus1.46±0.27?pg/μgfor 7 and 41 patients with and without invasion, resp). The ELISA procedure is, thus, an interesting tool to ensure VEGF and bFGF levels in meningiomas and to test putative correlations with clinical parameters. It is, thus, tempting to speculate that ELISA would also be valuable for the quantitative analysis of other angiogenic growth factors and cytokines in intracranial tumours.
机译:使用ELISA对Ⅰ类脑膜瘤的各种亚型中的VEGF进行定量分析,发现脑膜内皮中的VEGF含量更高(2.38±0.62?pg /μg蛋白,n = 7),过渡型(1.08±0.21?pg /μg蛋白,n = 13)和微囊性脑膜瘤(1.98±0.87?pg /μg蛋白质,n = 5),而纤维性脑膜瘤(0.36±0.09?pg /μg蛋白质,n = 5)。与VEGF相反,未检测到bFGF浓度的差异。 VEGF水平与脑膜瘤​​等级(分别为32和16级I和II,分别为1.47±0.23?pg /μg,2.29±0.58?pg /μg,2.29±0.58?pg /μg),血管形成(1.53±0.41?pg /μgversus1.96±0.28?pg /μg适用于24个低和24个高血管化肿瘤(分别)和脑浸润(2.32±0.59?pg /μgversus1.46±0.27?pg /μg适用于7个和41个有或没有浸润的患者,分别)。因此,ELISA程序是确保脑膜瘤中VEGF和bFGF水平并测试与临床参数相关性的有趣工具。因此,很容易推测ELISA对于颅内肿瘤中其他血管生成生长因子和细胞因子的定量分析也很有价值。

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