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首页> 美国卫生研究院文献>Gut >Cysteamine protects gastric epithelial cell monolayers against drug induced damage: evidence for direct cellular protection by sulphydryl compounds.
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Cysteamine protects gastric epithelial cell monolayers against drug induced damage: evidence for direct cellular protection by sulphydryl compounds.

机译：半胱胺可保护胃上皮细胞单层免受药物引起的损害：硫代化合物可直接对细胞进行保护。

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摘要

The sulphydryl containing drug cysteamine protects gastric mucosa in vivo against acute injury. It is not known whether this protection includes a direct effect on gastric cells. Using gastric epithelial cell monolayers derived from a well differentiated human cell line, we evaluated whether cysteamine protects against taurocholate or indomethacin induced damage in conditions which completely exclude the influence of vascular, hormonal, and neural factors. The effect of cysteamine on prostaglandin production by monolayer cells in vitro was also assessed. Cysteamine decreased damage brought about by sodium taurocholate and indomethacin by 40% (p less than 0.01) and 50% (p less than 0.01) respectively. The sulphydryl blocker iodoacetamide prevented the protective effect of cysteamine. Pretreatment with indomethacin, which inhibited prostaglandin E2 output by 60%, did not prevent protection by cysteamine; incubation with cysteamine decreased prostaglandin E2 production by cultured cells. We conclude that (i) cysteamine directly protected gastric epithelial cells in vitro (ii) this protection occurred with indomethacin, which interferes with cellular metabolism of prostaglandins, and taurocholate, whose damaging action at neutral pH is unrelated to interference with prostanoid metabolism, (iii) cysteamine protection in vitro is unrelated to endogenous prostaglandins and is probably mediated by endogenous sulphydryl compounds.
机译：含有巯基半胱胺的巯基保护体内的胃粘膜免受急性损伤。尚不清楚这种保护是否包括对胃细胞的直接作用。使用源自分化良好的人类细胞系的胃上皮细胞单层，我们评估了半胱胺是否能在完全排除血管，激素和神经因素影响的条件下预防牛磺酸胆甾醇或吲哚美辛诱导的损伤。还评估了半胱胺对体外单层细胞产生前列腺素的影响。半胱胺可使牛磺胆酸钠和消炎痛引起的伤害分别降低40％（p小于0.01）和50％（p小于0.01）。巯基阻断剂碘乙酰胺阻止了半胱胺的保护作用。消炎痛预处理可抑制前列腺素E2的输出60％，但不能阻止半胱胺的保护。与半胱胺一起孵育可降低培养细胞产生的前列腺素E2含量。我们得出的结论是（i）半胱胺可在体外直接保护胃上皮细胞（ii）吲哚美辛会干扰前列腺素和牛磺胆酸盐的作用，吲哚美辛会干扰前列腺素的细胞代谢，牛磺胆酸盐在中性pH下的破坏作用与前列腺素的代谢无关。（iii ）半胱胺的体外保护与内源性前列腺素无关，可能是由内源性巯基化合物介导的。

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