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首页> 美国卫生研究院文献>Genome Research >A Cattle–Human Comparative Map Built with Cattle BAC-Ends and Human Genome Sequence
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A Cattle–Human Comparative Map Built with Cattle BAC-Ends and Human Genome Sequence

机译：用牛BAC末端和人建立的牛人比较图 基因组序列

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摘要

As a step toward the goal of adding the cattle genome to those available for multispecies comparative genome analysis, 40,224 cattle BAC clones were end-sequenced, yielding 60,547 sequences (BAC end sequences, BESs) after trimming with an average read length of 515 bp. Cattle BACs were anchored to the human and mouse genome sequences by BLASTN search, revealing 29.4% and 10.1% significant hits (E < e-5), respectively. More than 60% of all cattle BES hits in both the human and mouse genomes are located within known genes. In order to confirm in silico predictions of orthologyand their relative position on cattle chromosomes, 84 cattle BESs with similarity to sequences on HSA11 were mapped using a cattle–hamster radiation hybrid (RH) panel. Resulting RH maps of BTA15 and BTA29 cover ∼85% of HSA11 sequence, revealing a complex patchwork shuffling of segments not explained by a simple translocation followed by internal rearrangements. Overlay of the mouse conserved syntenies onto HSA11 revealed that segmental boundaries appear to be conserved in all three species. The BAC clone-based comparative map provides a foundation for the evolutionary analysis of mammalian karyotypes and for sequencing of the cattle genome.
机译：为了朝着将牛基因组添加到可用于多物种比较基因组分析的目标中迈出的一步，对40,224个牛BAC克隆进行了末端测序，在修剪后产生60,547个序列（BAC末端序列，BES），平均读取长度为515 bp。通过BLASTN搜索将牛BAC锚定在人和小鼠基因组序列上，分别显示29.4％和10.1％的显着命中率（E -5 ）。人类和小鼠基因组中所有牛BES命中的60％以上都位于已知基因内。为了确认在计算机学上对正交学及其在牛染色体上的相对位置的预测，使用牛-仓鼠辐射杂交（RH）面板绘制了84个与HSA11序列相似的牛BES。所得的BTA15和BTA29的RH图谱覆盖了〜85％的HSA11序列，揭示了片段的复杂拼凑改组，这不能通过简单的易位然后内部重排来解释。小鼠保守同系物在HSA11上的重叠显示，在所有三个物种中节段边界似乎都是保守的。基于BAC克隆的比较图谱为哺乳动物核型的进化分析和牛基因组测序提供了基础。

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